Free Radical-Based Sequencing for Native Top-Down Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00252
Abstract: Native top-down proteomics allows for both proteoform identification and high-order structure characterization for cellular protein complexes. Unfortunately, tandem MS-based fragmentation efficiencies for such targets are low due to an increase in analyte ion mass and… read more here.
Keywords: native top; mass spectrometry; free radical; mass ... See more keywords
A Conformation-Specific Approach to Native Top-down Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.4c00361
Abstract: Native top-down mass spectrometry is a powerful approach for characterizing proteoforms and has recently been applied to provide similarly powerful insights into protein conformation. Current approaches, however, are limited such that structural insights can only… read more here.
Keywords: mass spectrometry; approach; conformation; native top ... See more keywords
Uncovering hidden protein modifications with native top-down mass spectrometry
Sign Up to like & getrecommendations! Published in 2025 at "Nature Methods"
DOI: 10.1038/s41592-025-02846-5
Abstract: Protein modifications drive dynamic cellular processes by modulating biomolecular interactions, yet capturing these modifications within their native structural context remains a significant challenge. Native top-down mass spectrometry promises to preserve the critical link between modifications… read more here.
Keywords: mass spectrometry; top mass; protein modifications; native top ... See more keywords