Identification of Editable Sites, Spacer Oligonucleotide Design, and Generation of the Gene-Targeting CRISPR-nCas9 Plasmid for Gene Disruption in Staphylococcus aureus Using the CRISPR-nCas9 and Cytidine Deaminase System.
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DOI: 10.1101/pdb.prot107924
Abstract: Methods for gene disruption are essential for functional genomics, and there are multiple approaches for altering gene function in bacteria. One of these methods involves introducing a premature stop codon in a gene of interest,… read more here.
Keywords: ncas9 cytidine; gene targeting; cytidine deaminase; gene ... See more keywords