The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Microbiological Methods"
DOI: 10.1016/j.mimet.2021.106323
Abstract: Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application… read more here.
Keywords: cupriavidus; necator h16; pmtl70000 modular; cupriavidus necator ... See more keywords
pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16.
Sign Up to like & getrecommendations! Published in 2021 at "New biotechnology"
DOI: 10.1016/j.nbt.2021.07.003
Abstract: Cupriavidus necator H16 is a chemolithoautotroph with a range of industrial biotechnological applications. Advanced metabolic engineering in the bacterium, however, is impeded by low transformation efficiency, making it difficult to introduce and screen new genetic… read more here.
Keywords: necator h16; cupriavidus necator; pcat vectors;
Metabolic engineering of Cupriavidus necator H16 for improved chemoautotrophic growth and PHB production under oxygen-limiting conditions.
Sign Up to like & getrecommendations! Published in 2020 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2020.04.009
Abstract: The oxygen-limiting condition promotes the accumulation of ployhydroxybutyrate (PHB) in C. necator H16, while the growth of which is restricted. Under autotrophic culture using carbon dioxide, hydrogen, and oxygen as substrates, the oxygen concentration below… read more here.
Keywords: oxygen limiting; necator h16; phb; limiting conditions ... See more keywords
The Overexpression of Phasin and Regulator Genes Promoting the Synthesis of Polyhydroxybutyrate in Cupriavidus necator H16 under Nonstress Conditions
Sign Up to like & getrecommendations! Published in 2021 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01458-21
Abstract: Microbial fixation of CO2 is an effective way to reduce greenhouse gases. Some microbes, such as C. necator H16, usually accumulate PHB when they grow under stress. ABSTRACT Cupriavidus necator H16 is an ideal strain… read more here.
Keywords: phb synthesis; h16; necator h16;
Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers.
Sign Up to like & getrecommendations! Published in 2019 at "Canadian journal of microbiology"
DOI: 10.1139/cjm-2018-0335
Abstract: We have characterized the ability of eight bacterial strains to utilize powdered low-density polyethylene (LDPE) plastic (untreated and without any additives) as a sole carbon source. Cell mass production on LDPE-containing medium after 21 days… read more here.
Keywords: polyhydroxyalkanoate polymers; necator h16; polyhydroxyalkanoate; density polyethylene ... See more keywords
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-019-1489-5
Abstract: Background3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacterial species and other… read more here.
Keywords: metabolism cupriavidus; necator; necator h16; cupriavidus necator ... See more keywords
A genome-scale metabolic model of Cupriavidus necator H16 integrated with TraDIS and transcriptomic data reveals metabolic insights for biotechnological applications
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1010106
Abstract: Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis… read more here.
Keywords: necator h16; cupriavidus necator; model; genome scale ... See more keywords