The bacterial cytosine deaminase gene used as a conditional negative selection marker in transgenic rice plants
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s002990100390
Abstract: Abstract. The bacterial gene cod A encodes cytosine deaminase (CD), which converts 5-fluorocytosine (5-FC) to 5-fluorouracil (5-FU), a compound that is toxic to cell growth. Consequently, CD can potentially be used as a conditional negative selection… read more here.
Keywords: conditional negative; used conditional; rice; negative selection ... See more keywords
Lysine Deacetylase Substrate Selectivity: Distinct Interaction Surfaces Drive Positive and Negative Selection for Residues Following Acetyllysine
Sign Up to like & getrecommendations! Published in 2023 at "Biochemistry"
DOI: 10.1021/acs.biochem.3c00001
Abstract: Lysine acetylation is a post-translational modification that is reversed by lysine deacetylases (KDACs). The goal of this work was to identify determinants of substrate specificity for KDACs, focusing on short-range interactions occurring with residues immediately… read more here.
Keywords: negative selection; interaction; following acetyllysine; substrate ... See more keywords
Quantification of frequency-dependent genetic architectures in 25 UK Biobank traits reveals action of negative selection
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-08424-6
Abstract: Understanding the role of rare variants is important in elucidating the genetic basis of human disease. Negative selection can cause rare variants to have larger per-allele effect sizes than common variants. Here, we develop a… read more here.
Keywords: frequency; negative selection; selection; genetic architectures ... See more keywords
Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10525-1
Abstract: During thymic negative selection, autoreactive thymocytes carrying T cell receptor (TCR) with overtly strong affinity to self-MHC/self-peptide are removed by Bim-dependent apoptosis, but how Bim is specifically regulated to link TCR activation and apoptosis induction… read more here.
Keywords: negative selection; thymic negative; apoptosis induction; apoptosis ... See more keywords
Evolutionary dynamics of neoantigens in growing tumors
Sign Up to like & getrecommendations! Published in 2020 at "Nature genetics"
DOI: 10.1038/s41588-020-0687-1
Abstract: Cancers accumulate mutations that lead to neoantigens, novel peptides that elicit an immune response, and consequently undergo evolutionary selection. Here we establish how negative selection shapes the clonality of neoantigens in a growing cancer by… read more here.
Keywords: negative selection; dynamics neoantigens; selection; immune escape ... See more keywords
A TCR mechanotransduction signaling loop induces negative selection in the thymus
Sign Up to like & getrecommendations! Published in 2018 at "Nature Immunology"
DOI: 10.1038/s41590-018-0259-z
Abstract: The T cell antigen receptor (TCR) expressed on thymocytes interacts with self-peptide major histocompatibility complex (pMHC) ligands to signal apoptosis or survival. Here, we found that negative-selection ligands induced thymocytes to exert forces on the… read more here.
Keywords: negative selection; selection; pmhc cd8; pmhc ... See more keywords
Influence of correlated antigen presentation on T-cell negative selection in the thymus
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the Royal Society Interface"
DOI: 10.1098/rsif.2018.0311
Abstract: The thymus is the primary organ for the generation of naive T cells, a key component of the immune system. Tolerance of T cells to self is achieved primarily in the thymic medulla, where immature… read more here.
Keywords: influence correlated; negative selection; selection; antigen presentation ... See more keywords
Positive selection of immune repertoires: A short further history
Sign Up to like & getrecommendations! Published in 2022 at "Scandinavian Journal of Immunology"
DOI: 10.1111/sji.13144
Abstract: The importance of the negative selection of self‐reacting cells from immune repertoires was easily recognized since it would militate against autoimmune disease. However, while the existence of positive selection in the auditioning of newly formed… read more here.
Keywords: negative selection; selection immune; positive selection; immune repertoires ... See more keywords
Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species
Sign Up to like & getrecommendations! Published in 2023 at "mSphere"
DOI: 10.1128/msphere.00682-22
Abstract: Multiple medically significant Streptococcus species, such as S. mutans, have highly sophisticated genetic systems available, largely as a consequence of their amenability to genetic manipulation via natural competence. Despite this, few options are available for… read more here.
Keywords: negative selection; system; mutagenesis; streptococcus ... See more keywords
Genomic Features and Pervasive Negative Selection in Rhodanobacter Strains Isolated from Nitrate and Heavy Metal Contaminated Aquifer
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02591-21
Abstract: Despite the dominance of Rhodanobacter species in the subsurface of the contaminated Oak Ridge Reservation (ORR) site, very little is known about the mechanisms underlying their adaptions to the various stressors present at ORR. Recently,… read more here.
Keywords: negative selection; rhodanobacter strains; rhodanobacter; strains isolated ... See more keywords
A bacterial dual positive and negative selection system for dCas9 activity
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0269270
Abstract: The engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent… read more here.
Keywords: negative selection; system; dcas9; dcas9 activity ... See more keywords