Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation
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DOI: 10.7554/elife.81086
Abstract: Targeted selection-based genome-editing approaches have enabled many fundamental discoveries and are used routinely with high precision. We found, however, that replacement of DBP1 with a common selection cassette in budding yeast led to reduced expression… read more here.
Keywords: cassette; neighboring gene; expression; expression cassettes ... See more keywords