Nested PCR for Suttonella ornithocola reveals widespread infection in British Paridae species
Sign Up to like & getrecommendations! Published in 2017 at "European Journal of Wildlife Research"
DOI: 10.1007/s10344-017-1105-6
Abstract: Suttonella ornithocola, a bacterium in the Cardiobacteriaceae family, is postulated to act as a pathogen targeting the respiratory tract of wild birds in the tit families (Paridae and Aegithalidae). This organism has fastidious culture requirements,… read more here.
Keywords: ornithocola reveals; infection; nested pcr; pcr suttonella ... See more keywords
Bioassay-based detection of Toxoplasma gondii in free-range chickens from Khorramabad, Iran: comparison of direct microscopy and semi-nested PCR
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biology Reports"
DOI: 10.1007/s11033-020-05539-8
Abstract: This study aimed to investigate the occurrence of anti-Toxoplasma gondii antibodies in free-range chickens from Khorramabad, western Iran, and also to compare the performance of direct microscopy and semi-nested PCR in mice bioassayed with tissues… read more here.
Keywords: direct microscopy; semi nested; free range; microscopy ... See more keywords
Molecular Detection and Differentiation of Different Theileria Species in Naturally Infected Goats Using Nested PCR-RFLP: A First Report from Northern India.
Sign Up to like & getrecommendations! Published in 2022 at "Acta parasitologica"
DOI: 10.1007/s11686-022-00553-1
Abstract: PURPOSE Theileriosis is an economically important tick-borne pathogen with a serious impact on livestock health and productivity. Despite the fact that bovine theileriosis has been widely investigated, there exists a paucity of information on these… read more here.
Keywords: pcr rflp; different theileria; rflp; goats using ... See more keywords
A nested-PCR strategy for molecular diagnosis of mollicutes in uncultured biological samples from cows with vulvovaginitis.
Sign Up to like & getrecommendations! Published in 2018 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2017.11.018
Abstract: Bacteria classified in Mycoplasma (M. bovis and M. bovigenitalium) and Ureaplasma (U. diversum) genera are associated with granular vulvovaginitis that affect heifers and cows at reproductive age. The traditional means for detection and speciation of… read more here.
Keywords: strategy molecular; strategy; molecular diagnosis; pcr strategy ... See more keywords
Cryptosporidium spp. in caged exotic psittacines from Brazil: Evaluation of diagnostic methods and molecular characterization.
Sign Up to like & getrecommendations! Published in 2018 at "Experimental parasitology"
DOI: 10.1016/j.exppara.2017.12.004
Abstract: The aim of this study was to evaluate the prevalence of and diagnostic methods for Cryptosporidium spp. in caged adult exotic parrots from Southern and Southeastern Brazil. Oocysts were purified from fecal samples from 463… read more here.
Keywords: nested pcr; genotype; cryptosporidium; cryptosporidium spp ... See more keywords
An autopsy case of tuberculous meningitis undiagnosed by nested-PCR of CSF samples and brain biopsy
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the Neurological Sciences"
DOI: 10.1016/j.jns.2020.116968
Abstract: Nested polymerase chain reaction (PCR) testing of cerebrospinal fluid (CSF) has higher diagnostic sensitivity with regard to tuberculous meningitis (TBM) than conventional methods. Herein we describe the autopsy case of a 70-year-old woman with TBM… read more here.
Keywords: case; csf; pcr csf; brain biopsy ... See more keywords
Leishmania species in biopsies of patients with different clinical manifestations identified by high resolution melting and nested PCR in an Endemic district in Peru
Sign Up to like & getrecommendations! Published in 2019 at "Parasite Epidemiology and Control"
DOI: 10.1016/j.parepi.2019.e00095
Abstract: Background The parasite of the genus Leishmania causes a neglected disease known as leishmaniasis, which has different clinical aspects depending on the species that infects the person and the immune response of the patient. The… read more here.
Keywords: district; different clinical; clinical manifestations; leishmania ... See more keywords
Detection and characterization of Cryptosporidium species and genotypes in three chicken production systems in Brazil using different molecular diagnosis protocols.
Sign Up to like & getrecommendations! Published in 2018 at "Preventive veterinary medicine"
DOI: 10.1016/j.prevetmed.2018.01.007
Abstract: The objective of this study was to determine the occurrence of Cryptosporidium spp. in domestic chickens raised in different chicken production systems in Brazil using three nested PCR protocols. The purification and concentration of oocysts… read more here.
Keywords: chicken production; cryptosporidium; gene; production systems ... See more keywords
Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein
Sign Up to like & getrecommendations! Published in 2021 at "Water Research"
DOI: 10.1016/j.watres.2021.117104
Abstract: New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (∼1500 bps) to detect multiple nucleotide changes resulting in key spike… read more here.
Keywords: cov variants; spike protein; sars cov; pcr assays ... See more keywords
Development of a nested-PCR assay for the rapid detection of Pilidiella granati in pomegranate fruit
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep40954
Abstract: Pilidiella granati, a causal agent of twig blight and crown rot of pomegranate, is an emerging threat that may cause severe risk to the pomegranate industry in the future. Development of a rapid assay for… read more here.
Keywords: pomegranate; detection; granati; pilidiella granati ... See more keywords
Detection of Herpesvirus anguillae (AngHV-1) in European eel Anguilla anguilla (L.) originating from northern Poland-assessment of suitability of selected diagnostic methods.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of fish diseases"
DOI: 10.1111/jfd.12689
Abstract: The Community Action Plan requests EU member states to implement measures that ensure the recovery of the severely depleted European eel stocks. One of the main threats is posed by Anguillid herpesvirus 1 (AngHV-1) leading… read more here.
Keywords: anguilla; diagnostic methods; pcr; european eel ... See more keywords