Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand–Analyte Interactions
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DOI: 10.1021/acsomega.1c05659
Abstract: Quantitative analysis of protein–protein interactions (PPIs) using biolayer interferometry (BLI) requires effective suppression of nonspecific binding (NSB) between analytes and biosensors. In particular, the study of weak interactions (i.e., KD > 1 μM) requires high… read more here.
Keywords: weak interactions; biolayer interferometry; new nsb; nonspecific binding ... See more keywords