An Optimized, CE‐Compatible, Targeted NGS‐Based SSR Genotyping Method Using Primer‐Anchored Alignment
Sign Up to like & getrecommendations! Published in 2025 at "ELECTROPHORESIS"
DOI: 10.1002/elps.70007
Abstract: Microsatellites (SSRs) are highly polymorphic DNA sequences widely used in genetic research, including parentage assignment. Traditional SSR analysis relies on capillary electrophoresis (CE), which is time‐consuming and has limited capacity. Next‐generation sequencing (NGS) offers a… read more here.
Keywords: method; ngs based; ssr genotyping; optimized ssrseq ... See more keywords
Development and evaluation of a rapid and cost-efficient NGS-based MHC class I genotyping method for macaques by using a prevalent short-read sequencer.
Sign Up to like & getrecommendations! Published in 2021 at "Immunogenetics"
DOI: 10.1007/s00251-020-01199-x
Abstract: Rhesus macaque is one of the most widely used primate model animals for immunological research of infectious diseases including human immunodeficiency virus (HIV) infection. It is well known that major histocompatibility complex (MHC) class I… read more here.
Keywords: short read; method; ngs based; genotyping method ... See more keywords
Clinical application of an NGS-based method in the preimplantation genetic testing for Duchenne muscular dystrophy
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-021-02126-z
Abstract: To determine whether next-generation sequencing (NGS) could be used to directly detect different mutations of Duchenne muscular dystrophy (DMD) during preimplantation genetic testing (PGT). From Sep. 2016 to Aug. 2018, a total of six couples… read more here.
Keywords: genetic testing; muscular dystrophy; preimplantation; ngs based ... See more keywords
The utility and perspectives of NGS-based methods in BSL-3 and BSL-4 laboratory – sequencing and analysis strategies
Sign Up to like & getrecommendations! Published in 2018 at "Briefings in Functional Genomics"
DOI: 10.1093/bfgp/elx033
Abstract: Abstract Modern diagnostics is in general based on molecular biology methods. Nowadays sequencing-based methods, especially whole genome sequencing, are becoming increasingly important. Implementation of such methods into routine diagnostic of highly dangerous pathogens, like Bacillus… read more here.
Keywords: perspectives ngs; utility perspectives; bsl laboratory; ngs based ... See more keywords
P-545 Early pregnancy outcomes associated with age and AMH following Next-Generation Sequencing(NGS)-based Preimplantation Genetic Testing for Aneuploidy(PGT-A) in single euploid embryo transfers for advanced age patients
Sign Up to like & getrecommendations! Published in 2024 at "Human Reproduction"
DOI: 10.1093/humrep/deae108.884
Abstract: Does age and AMH impact early pregnancy outcomes in euploid single embryo transfers using NGS-based PGT-A for individuals aged 35 and above? In this NGS-based PGT-A study, no significant differences in early pregnancy rates were… read more here.
Keywords: age amh; age; ngs based; pregnancy ... See more keywords
Recommendations to improve the clinical adoption of NGS‐based cancer diagnostics in Singapore
Sign Up to like & getrecommendations! Published in 2020 at "Asia-Pacific Journal of Clinical Oncology"
DOI: 10.1111/ajco.13339
Abstract: Next‐generation sequencing (NGS)‐based diagnostics have demonstrated clinical utility in predicting improved survival benefits with targeted treatment in certain cancer types, and positive cost–benefit in several healthcare systems. However, clinical adoption in Singapore remains low despite… read more here.
Keywords: clinical adoption; ngs based; adoption ngs; cancer ... See more keywords
Abstract 4315: NGS-based reference materials for fusion and somatic variant detection in myeloid cancers
Sign Up to like & getrecommendations! Published in 2020 at "Endocrinology"
DOI: 10.1158/1538-7445.am2020-4315
Abstract: The large range of genetic aberrations and genes involved Myeloid malignancies, clonal diseases of hematopoietic progenitor cells which can lead to accumulation of immature blast cells in the bone marrow and peripheral blood, make Next… read more here.
Keywords: detection; reference materials; rna; reference ... See more keywords
Abstract LB095: ASPYRE-Lung addresses critical gaps in NGS-based biomarker testing: Robust variant calling from NGS QC fails
Sign Up to like & getrecommendations! Published in 2024 at "Cancer Research"
DOI: 10.1158/1538-7445.am2024-lb095
Abstract: Advanced NSCLC treatment guidelines recommend testing patients for genomic biomarkers to utilize over 30 FDA-approved targeted therapies. A major challenge with current NGS-based testing methods is that ~45% of patient samples fail NGS quality control… read more here.
Keywords: lung; ngs based; aspyre lung; biomarker ... See more keywords
NGS-Based Immunoglobulin Gene Sequencing for Diagnosis and MRD Monitoring of Multiple Myeloma
Sign Up to like & getrecommendations! Published in 2024 at "Blood"
DOI: 10.1182/blood-2024-209254
Abstract: Multiple myeloma (MM) is a malignant plasma cell disorder characterized by the clonal proliferation of malignant plasma cells in the bone marrow, leading to bone lesions, anemia, hypercalcemia, renal insufficiency, and immunodeficiency. Accurate diagnosis and… read more here.
Keywords: diagnosis; ngs based; rna seq; mrd ... See more keywords
Clinical utility of next-generation sequencing (NGS)-based genomic profiling of advanced solid tumors in routine clinical practice.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2018.36.15_suppl.e24157
Abstract: e24157Background: Next-generation sequencing (NGS)-based genomic profiling of advanced solid tumors has given a new perspective to personalized patient care in oncology. In a single run, NGS allows... read more here.
Keywords: sequencing ngs; ngs based; next generation; oncology ... See more keywords
NGS-Based BRCA1, BRCA2, and PALB2 Mutation Testing in Iranian Population With Breast Cancer
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Global Oncology"
DOI: 10.1200/jgo.18.84100
Abstract: Background: Identification of individuals who have a pathogenic mutation in breast cancer susceptibility genes is an important step to take advantage of genetic counseling, screening, and potential... read more here.
Keywords: based brca1; ngs based; mutation; breast cancer ... See more keywords