Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
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DOI: 10.1021/jacs.8b05098
Abstract: N-Hydroxysuccinimide (NHS)-esters are widely used to label proteins nonselectively on free amino groups. Such broad labeling can be disadvantageous because it can interfere with protein structure or function and because stoichiometry is poorly controlled. Here… read more here.
Keywords: protein; site specific; protein labeling; ubiquitin ligase ... See more keywords