Protein encapsulation in the hollow space of hemocyanin crystals containing a covalently conjugated ligand.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.04.062
Abstract: Encapsulation of guest molecules into the vacant space of biomacromolecular crystals has been utilized for various purposes including functioning as a protein container to protect against physical stress and structural determination of the guest. Todarodes… read more here.
Keywords: protein; space; conjugated ligand; tphc ... See more keywords
Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein
Sign Up to like & getrecommendations! Published in 2019 at "European Polymer Journal"
DOI: 10.1016/j.eurpolymj.2019.01.024
Abstract: Abstract We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface… read more here.
Keywords: immobilization; his6 protein; fe3o4 ni2; ni2 nta ... See more keywords