An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplification.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.03.013
Abstract: An ultra-sensitive aptamer-based biosensor for the detection of staphylococcus aureus was established by adopting the nicking enzyme amplification reaction (NEAR) and the rolling circle amplification (RCA) technologies. Aptamer-probe (AP), containing an aptamer and a probe… read more here.
Keywords: staphylococcus aureus; enzyme amplification; amplification reaction; nicking enzyme ... See more keywords
Label-free and nicking enzyme-assisted fluorescence signal amplification for RNase H determination based on a G-quadruplexe/thioflavin T complex.
Sign Up to like & getrecommendations! Published in 2018 at "Talanta"
DOI: 10.1016/j.talanta.2018.01.075
Abstract: In this paper, we describe a novel, label-free and nicking enzyme-assisted fluorescence signal amplification strategy that demonstrates to be cost efficient, sensitive, and unique for assaying the RNase H activity and inhibition based on G-quadruplex… read more here.
Keywords: enzyme assisted; rnase; enzyme; nicking enzyme ... See more keywords
Fluorescent Assay of FEN1 Activity with Nicking Enzyme-Assisted Signal Amplification Based on ZIF-8 for Imaging in Living Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c05473
Abstract: Flap endonuclease 1 (FEN1) participates in both DNA replication and repair to maintain the stability and integrity of the genome. As a potential tumor marker, detecting FEN1 activity could be an effective strategy for cancer… read more here.
Keywords: assay fen1; fen1 activity; nicking enzyme; living cells ... See more keywords
Types and Applications of Nicking Enzyme-Combined Isothermal Amplification
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23094620
Abstract: Due to the sudden outbreak of COVID-19 at the end of 2019, rapid detection has become an urgent need for community clinics and hospitals. The rapid development of isothermal amplification detection technology for nucleic acids… read more here.
Keywords: enzyme combined; amplification; nicking enzyme; isothermal amplification ... See more keywords