Identification of two subtilisin‐like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana
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DOI: 10.1002/1873-3468.14014
Abstract: The tobacco variant Nicotiana benthamiana has recently emerged as a versatile host for the manufacturing of protein therapeutics, but the fidelity of many recombinant proteins generated in this system is compromised by inadvertent proteolysis. Previous… read more here.
Keywords: serine proteases; subtilisin like; like serine; recombinant proteins ... See more keywords
Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana
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DOI: 10.1002/biot.202000566
Abstract: Human angiotensin‐converting enzyme 2 (ACE2) is the primary host cell receptor for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) binding and cell entry. Administration of high concentrations of soluble ACE2 can be utilized as a… read more here.
Keywords: glycoengineered nicotiana; ace2; receptor; sars cov ... See more keywords
CRISPR/Cas9-mediated knockout of a prolyl-4-hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection.
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DOI: 10.1002/biot.202100698
Abstract: The properties of host plants used for molecular farming can be modified by CRISPR/Cas9 genome editing to improve the quality and yield of recombinant proteins. However, it is often necessary to target multiple genes simultaneously,… read more here.
Keywords: nicotiana benthamiana; prolyl hydroxylase; crispr cas9; plant ... See more keywords
Efficient Establishment of Interfamily Heterograft of Nicotiana benthamiana and Arabidopsis thaliana.
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DOI: 10.1007/978-1-4939-9562-2_31
Abstract: The grafting technique has been applied to study systemic signaling in plants, especially to investigate whether gene action is graft transmissible and/or gene products such as RNAs and proteins are transported systemically. Here we describe… read more here.
Keywords: heterograft nicotiana; establishment interfamily; interfamily heterograft; efficient establishment ... See more keywords
Transient overexpression of E2Fb triggers cell divisions in pavement cells of Nicotiana benthamiana leaves
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DOI: 10.1007/s00299-019-02457-3
Abstract: Agrobacterium-mediated transient overexpression of E2Fb triggers new cell divisions in pavement cells of Nicotiana benthamiana leaves. Transient transformation in Nicotiana benthamiana enables the study of multiple biological processes in a simple and fast manner. Here,… read more here.
Keywords: pavement cells; cell divisions; benthamiana; transient overexpression ... See more keywords
Nicotiana benthamiana NbbZIP28, a possible regulator of unfolded protein response, plays a negative role in viral infection
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DOI: 10.1007/s10658-017-1231-8
Abstract: The unfolded protein response (UPR), a highly conserved cellular defense pathway, is activated by endoplasmic reticulum (ER) stress. In Arabidopsis, the transcription factor AtbZIP28 mediates ER stress responses induced by tunicamycin. Here, we identified NbbZIP28… read more here.
Keywords: nbbzip28; response; unfolded protein; protein response ... See more keywords
The sugarcane ShMYB78 transcription factor activates suberin biosynthesis in Nicotiana benthamiana
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DOI: 10.1007/s11103-020-01048-1
Abstract: A sugarcane MYB present in the culm induces suberin biosynthesis and is involved both with fatty acid and phenolics metabolism. Few transcription factors have been described as regulators of cell wall polymers deposition in C4… read more here.
Keywords: transcription; biosynthesis; suberin biosynthesis; sugarcane ... See more keywords
Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana
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DOI: 10.1007/s11240-020-01838-x
Abstract: Mambalgin-1 is a peptide that acts as a potent analgesic through inhibiting acid-sensing ion channels (ASIC) in nerve cells. Research has shown that ASIC channels are involved in the proliferation and growth of cancer cells;… read more here.
Keywords: mambalgin peptide; mambalgin; cancer cells; cancer ... See more keywords
The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana
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DOI: 10.1007/s13337-017-0413-5
Abstract: Abstract Alphasatellites are non-essential satellite-like components associated with geminiviruses. The precise selective advantage to a geminivirus infection of an alphasatellite remains unclear. The ability of the cotton leaf curl Multan alphasatellite (CLCuMuA)-encoded replication-associated protein (Rep)… read more here.
Keywords: silenced green; tgs; transcriptionally silenced; expression ... See more keywords
Purification and site-specific N-glycosylation analysis of human recombinant butyrylcholinesterase from Nicotiana benthamiana
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DOI: 10.1016/j.bej.2018.11.004
Abstract: Abstract Butyrylcholinesterase (BChE) is a glycosylated serine hydrolase found in human serum that has been shown to protect against various cholinesterase-inhibiting organophosphate nerve agents. The supply of plasma-derived butyrylcholinesterase (hBChE) is constrained by the availability… read more here.
Keywords: purification; specific glycosylation; nicotiana benthamiana; butyrylcholinesterase ... See more keywords
Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins.
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DOI: 10.1016/j.jbiosc.2017.02.019
Abstract: The production of recombinant proteins in plants has many advantages, including safety and reduced costs. However, this technology still faces several issues, including low levels of production. The repression of RNA silencing seems to be… read more here.
Keywords: dcl2 dcl4; type plants; benthamiana plants; recombinant proteins ... See more keywords