Exploring a broad spectrum nitrilase from moderately halophilic bacterium Halomonas sp. IIIMB2797 isolated from saline lake
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800168
Abstract: Nitrile hydrolyzing moderate halophilic bacterium Halomonas sp. IIIMB2797 was isolated from Sambhar Lake, India. Maximum cell biomass and nitrilase production were observed at 60 g L−1 NaCl in the production media which confirms its moderate halophilic nature.… read more here.
Keywords: bacterium halomonas; halophilic bacterium; nitrilase; halomonas iiimb2797 ... See more keywords
The use of clade‐specific PCR assays to identify novel nitrilase genes from environmental isolates
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DOI: 10.1002/mbo3.700
Abstract: Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio‐pharmaceuticals and industrial applications facilitates the move towards… read more here.
Keywords: novel nitrilase; nitrilase; clade specific; pcr ... See more keywords
Erratum to: Enhancing the catalytic potential of nitrilase from Pseudomonas putida for stereoselective nitrile hydrolysis
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8413-3
Abstract: (R)-mandelic acid was produced from racemic mandelonitrile using free and immobilized cells of Pseudomonas putida MTCC 5110 harbouring a stereoselective nitrilase. In addition to the optimization of culture conditions and medium components, an inducer feeding… read more here.
Keywords: erratum enhancing; enhancing catalytic; pseudomonas putida; nitrilase ... See more keywords
Highly regio- and enantioselective synthesis of chiral intermediate for pregabalin using one-pot bienzymatic cascade of nitrilase and amidase
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09857-1
Abstract: Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The… read more here.
Keywords: pregabalin; pot bienzymatic; nitrilase; bienzymatic cascade ... See more keywords
A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production
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DOI: 10.1007/s00449-017-1787-x
Abstract: A novel aliphatic nitrilase, REH16, was found in Ralstonia eutropha H16 and overexpressed in Escherichia coli BL21(DE3), and its enzymatic properties were studied. The temperature and pH optima were 37 °C and 6.6, respectively, and the… read more here.
Keywords: ralstonia eutropha; nitrilase; cyanopyridine; eutropha h16 ... See more keywords
Enhanced catalytic activity of new acryloyl crosslinked cellulose dialdehyde-nitrilase Schiff base and its reduced form for nitrile hydrolysis.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.03.034
Abstract: Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for… read more here.
Keywords: acryloyl crosslinked; new acryloyl; nitrilase; form ... See more keywords
Machine learning modelling for the ultrasonication-mediated disruption of recombinant E. coli for the efficient release of nitrilase.
Sign Up to like & getrecommendations! Published in 2019 at "Ultrasonics"
DOI: 10.1016/j.ultras.2019.06.006
Abstract: The ultrasonication-mediated cell disruption of recombinant E. coli was modeled using three machine learning techniques namely Multiple linear regression (MLR), Multi-layer perceptron (MLP) and Sequential minimal optimization (SMO). The four attributes were cellmass concentration (g/L),… read more here.
Keywords: disruption recombinant; disruption; nitrilase; recombinant coli ... See more keywords
Functional metagenomics uncovers nitrile-hydrolysing enzymes in a coal metagenome
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1123902
Abstract: Introduction: Nitriles are the most toxic compounds that can lead to serious human illness through inhalation and consumption due to environmental pollution. Nitrilases can highly degrade nitriles isolated from the natural ecosystem. In the current… read more here.
Keywords: nitrile hydrolysing; nitrilase; coal metagenome; metagenomics uncovers ... See more keywords
Novel Chaperones RrGroEL and RrGroES for Activity and Stability Enhancement of Nitrilase in Escherichia coli and Rhodococcus ruber
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DOI: 10.3390/molecules25041002
Abstract: For large-scale bioproduction, thermal stability is a crucial property for most industrial enzymes. A new method to improve both the thermal stability and activity of enzymes is of great significance. In this work, the novel… read more here.
Keywords: nitrilase; expression; rrgroel; stability ... See more keywords