Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac‐mandelonitrile by semi‐rational engineering
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26484
Abstract: Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high‐value chiral chemicals is often time‐, money‐, and resource‐intensive; traditional semi‐rational designs… read more here.
Keywords: semi rational; stereoselectivity; man; nitrile hydratase ... See more keywords
Analyzing the function of the insert region found between the α and β-subunits in the eukaryotic nitrile hydratase from Monosiga brevicollis.
Sign Up to like & getrecommendations! Published in 2018 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.08.013
Abstract: The functional roles of the (His)17 region and an insert region in the eukaryotic nitrile hydratase (NHase, EC 4.2.1.84) from Monosiga brevicollis (MbNHase), were examined. Two deletion mutants, MbNHaseΔ238-257 and MbNHaseΔ219-272, were prepared in which… read more here.
Keywords: eukaryotic nitrile; monosiga brevicollis; mbnhase; insert region ... See more keywords
Efficient biodegradation of dihalogenated benzonitrile herbicides by recombinant Escherichia coli harboring nitrile hydratase-amidase pathway
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2017.05.021
Abstract: Abstract Worldwide use of benzonitrile herbicides has caused a contamination hazard for groundwater, and their removal has attracted increasing attention. Microbial degradation has been considered as a major route of removing toxic nitriles from the… read more here.
Keywords: coli; benzonitrile herbicides; nitrile hydratase; hydratase amidase ... See more keywords
Nitrile hydratase mediated green synthesis of lactamide by immobilizing Rhodococcus pyridinivorans NIT-36 cells on N, N'-Methylene bis-acrylamide activated chitosan.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.06.004
Abstract: In this paper green synthesis of an important commodity chemical lactamide has been undertaken using chitosan immobilized Rhodococcus pyridinivorans NIT-36 harbouring nitrile hydratase (NHase) enzyme. The cells immobilization (300 mg/g) is based on the partial entrapment… read more here.
Keywords: pyridinivorans nit; rhodococcus pyridinivorans; green synthesis; lactamide ... See more keywords
Engineering of the thermophilic nitrile hydratase from Pseudonocardia thermophila JCM3095 for large-scale nicotinamide production based on sequence-activity relationships.
Sign Up to like & getrecommendations! Published in 2021 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2021.09.132
Abstract: The green biocatalyst nitrile hydratase (NHase) is able to bio-transform 3-cyanopyridine into nicotinamide. As the NHase reaction is exothermic, an enzyme with high activity and stability is needed for nicotinamide production. In this study, we… read more here.
Keywords: production; pseudonocardia thermophila; nitrile hydratase; nicotinamide production ... See more keywords
Discovery of the ATPase Activity of a Cobalt-Type Nitrile Hydratase Activator and Its Promoting Effect on Enzyme Maturation.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00167
Abstract: An activator protein and a metal ion are two factors known to be indispensable for the maturation of nitrile hydratase (NHase). Here, the third key factor, adenosine triphosphate (ATP), was identified to play an important… read more here.
Keywords: activator protein; atpase activity; activity; nitrile hydratase ... See more keywords
Co(III) Complexes with N2S3-Type Ligands as Structural/Functional Models for the Isocyanide Hydrolysis Reaction Catalyzed by Nitrile Hydratase.
Sign Up to like & getrecommendations! Published in 2018 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.6b02324
Abstract: It has been before reported that, in addition to hydration of nitriles, the Fe-type nitrile hydratase (NHase) also catalyzes the hydrolysis of tert-butylisocyanide ( tBuNC). In order to investigate the unique isocyanide hydrolysis by NHase,… read more here.
Keywords: iii; hydrolysis reaction; isocyanide hydrolysis; nitrile hydratase ... See more keywords
A nanobiocatalytic system based on the self-assembly of ferrous phosphate with a high regioselectivity nitrile hydratase.
Sign Up to like & getrecommendations! Published in 2022 at "Letters in applied microbiology"
DOI: 10.1093/lambio/ovac071
Abstract: Our previous study identified a novel nitrile hydratase (NHase) with remarkable biotransformation activity toward adipamide during the production of 5-cyanovaleramide (5-CVAM), an important intermediate of herbicide and chemical raw material. Nevertheless, free NHase will face… read more here.
Keywords: self assembly; nhase; fe3 po4; free nhase ... See more keywords