Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7540-2_13
Abstract: Recent progress in the technology of transcriptome-wide high-throughput sequencing has revealed that nonsense-mediated mRNA decay (NMD) targets ~10% of physiologic transcripts for the purpose of tuning gene expression in response to various environmental conditions. Regardless… read more here.
Keywords: decay nmd; phosphorylated upf1; mediated mrna; nmd targets ... See more keywords
High-resolution profiling of NMD targets in yeast reveals translational fidelity as a basis for substrate selection.
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DOI: 10.1261/rna.060541.116
Abstract: Nonsense-mediated mRNA decay (NMD) plays an important role in eukaryotic gene expression, yet the scope and the defining features of NMD-targeted transcripts remain elusive. To address these issues, we reevaluated the genome-wide expression of annotated… read more here.
Keywords: high resolution; regulated transcripts; nmd regulated; profiling nmd ... See more keywords
Translational repression of NMD targets by GIGYF2 and EIF4E2.
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DOI: 10.1371/journal.pgen.1009813
Abstract: Translation of messenger RNAs (mRNAs) with premature termination codons produces truncated proteins with potentially deleterious effects. This is prevented by nonsense-mediated mRNA decay (NMD) of these mRNAs. NMD is triggered by ribosomes terminating upstream of… read more here.
Keywords: gigyf2 eif4e2; targets gigyf2; translational repression; nmd targets ... See more keywords