DNP‐enhanced solid‐state NMR spectroscopy of active pharmaceutical ingredients
Sign Up to like & getrecommendations! Published in 2018 at "Magnetic Resonance in Chemistry"
DOI: 10.1002/mrc.4688
Abstract: Solid‐state NMR spectroscopy has become a valuable tool for the characterization of both pure and formulated active pharmaceutical ingredients (APIs). However, NMR generally suffers from poor sensitivity that often restricts NMR experiments to nuclei with… read more here.
Keywords: solid state; state nmr; nmr experiments; nmr spectroscopy ... See more keywords
NMR experiments for the rapid identification of P=O···H–X type hydrogen bonds in nucleic acids
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-017-0140-7
Abstract: Hydrogen bonds involving the backbone phosphate groups occur with high frequency in functional RNA molecules. They are often found in well-characterized tertiary structural motifs presenting powerful probes for the rapid identification of these motifs for… read more here.
Keywords: nucleic acids; hydrogen bonds; hydrogen; nmr experiments ... See more keywords
Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated π pulses
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-017-0153-2
Abstract: In multidimensional solution NMR experiments, π pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during π pulse execution result in severe… read more here.
Keywords: triply compensated; enhancing sensitivity; nmr; nmr experiments ... See more keywords
Selective observation of charge storing ions in supercapacitor electrode materials.
Sign Up to like & getrecommendations! Published in 2018 at "Solid state nuclear magnetic resonance"
DOI: 10.1016/j.ssnmr.2017.10.003
Abstract: Nuclear magnetic resonance (NMR) spectroscopy has emerged as a useful technique for probing the structure and dynamics of the electrode-electrolyte interface in supercapacitors, as ions inside the pores of the carbon electrodes can be studied… read more here.
Keywords: nmr experiments; electrode materials; ions supercapacitor; selective observation ... See more keywords
Suppressing 1H Spin Diffusion in Fast MAS Proton Detected Heteronuclear Correlation Solid-State NMR Experiments.
Sign Up to like & getrecommendations! Published in 2019 at "Solid state nuclear magnetic resonance"
DOI: 10.1016/j.ssnmr.2019.101636
Abstract: Fast magic angle spinning (MAS) and indirect detection by high gyromagnetic ratio (γ) nuclei such as proton or fluorine are increasingly utilized to obtain 2D heteronuclear correlation (HETCOR) solid-state NMR spectra of spin-1/2 nuclei by… read more here.
Keywords: solid state; fast mas; nmr experiments; spin diffusion ... See more keywords
On-the-Fly, Sample-Tailored Optimisation of NMR Experiments
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01767
Abstract: NMR experiments, indispensable to chemists in many areas of research, are often run with generic, unoptimized experimental parameters. This approach makes robust and automated acquisition on different samples and instruments extremely challenging. Here, we present… read more here.
Keywords: sample tailored; optimisation nmr; nmr experiments; fly sample ... See more keywords
From Angstroms to Nanometers: Measuring Interatomic Distances by Solid-State NMR.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical reviews"
DOI: 10.1021/acs.chemrev.1c00662
Abstract: Internuclear distances represent one of the main structural constraints in molecular structure determination using solid-state NMR spectroscopy, complementing chemical shifts and orientational restraints. Although a large number of magic-angle-spinning (MAS) NMR techniques have been available… read more here.
Keywords: solid state; nmr; state nmr; distance ... See more keywords
NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c05603
Abstract: We have used chemical shift perturbation (CSP) and saturation transfer difference (STD) NMR experiments to identify and characterize the binding of selected ligands to the receptor-binding domain (RBD) of the spike glycoprotein (S-protein) of the… read more here.
Keywords: receptor binding; nmr experiments; binding domain; sars cov ... See more keywords
NMR experiments for studies of dilute and condensed protein phases: Application to the phase-separating protein CAPRIN1.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b12208
Abstract: Intrinsically disordered proteins (IDPs) or regions of intrinsic disorder in otherwise folded proteins (IDRs) play important roles in many different biological processes, including formation of biological condensates via liquid-liquid phase separation. NMR spectroscopy is a… read more here.
Keywords: protein; phase separation; phase; experiments studies ... See more keywords
Relaxation-encoded NMR experiments for mixture analysis: REST and beer.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc03150e
Abstract: A new family of NMR experiments for mixture analysis (Relaxation-Encoded Selective TOCSY, REST) allows the extraction of component subspectra from mixtures. It uses isotropic mixing to label whole spin systems with the relaxation times (e.g.… read more here.
Keywords: relaxation; nmr experiments; experiments mixture; mixture analysis ... See more keywords
Troubleshooting Guide to Expressing Intrinsically Disordered Proteins for Use in NMR Experiments
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2018.00118
Abstract: Intrinsically disordered proteins (IDPs) represent a structural class of proteins that do not have a well-defined, 3D fold in solution, and often have little secondary structure. To characterize their function and molecular mechanism, it is… read more here.
Keywords: guide expressing; nmr experiments; structure; troubleshooting guide ... See more keywords