Probing the interaction of a quercetin bioconjugate with Bcl‐2 in living human cancer cells with in‐cell NMR spectroscopy
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Letters"
DOI: 10.1002/1873-3468.13250
Abstract: In‐cell NMR spectroscopy has emerged as a powerful technique for monitoring biomolecular interactions at an atomic level inside intact cells. However, current methodologies are inadequate at charting intracellular interactions of nonlabeled proteins and require their… read more here.
Keywords: human cancer; living human; nmr spectroscopy; cell nmr ... See more keywords
Studies on the Formation of Dienamine and Trienamine Intermediates by 1H NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2017 at "Asian Journal of Organic Chemistry"
DOI: 10.1002/ajoc.201600616
Abstract: Formation of dienamines and trienamines from common aminocatalysts and conjugated enals or dienals, respectively, have been monitored by 1H NMR spectroscopy. The protocol allows a fast, simple and direct comparison of various starting materials in the… read more here.
Keywords: dienamine trienamine; nmr spectroscopy; formation dienamine; spectroscopy ... See more keywords
Artificially Linked Ubiquitin Dimers Characterised Structurally and Dynamically by NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900146
Abstract: As one of the most prevalent post‐translational modifications in eukaryotic cells, ubiquitylation plays vital roles in many cellular processes, such as protein degradation, DNA metabolism, and cell differentiation. Substrate proteins can be tagged by distinct… read more here.
Keywords: artificially linked; dimers characterised; ubiquitin dimers; nmr spectroscopy ... See more keywords
In‐Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Nucleic Acid Chemistry"
DOI: 10.1002/cpnc.71
Abstract: In‐cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near‐to‐atomic resolution. In this… read more here.
Keywords: spectroscopy nucleic; nmr spectroscopy; cell nmr; spectroscopy ... See more keywords
DNP‐enhanced solid‐state NMR spectroscopy of active pharmaceutical ingredients
Sign Up to like & getrecommendations! Published in 2018 at "Magnetic Resonance in Chemistry"
DOI: 10.1002/mrc.4688
Abstract: Solid‐state NMR spectroscopy has become a valuable tool for the characterization of both pure and formulated active pharmaceutical ingredients (APIs). However, NMR generally suffers from poor sensitivity that often restricts NMR experiments to nuclei with… read more here.
Keywords: solid state; state nmr; nmr experiments; nmr spectroscopy ... See more keywords
Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0524-0_11
Abstract: Cell-free protein synthesis (CFPS) is an established method to produce recombinant proteins and has been used in a wide variety of applications. The use of CFPS has almost from the onset been favorably linked to… read more here.
Keywords: nmr spectroscopy; cell free; proteins nmr; protein synthesis ... See more keywords
Preparation of Caveolin-1 for NMR Spectroscopy Experiments.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0732-9_13
Abstract: Caveolin-1 is a 20.5 kDa integral membrane protein that is involved in a myriad of cellular processes including signal transduction, relieving mechano-stresses on the cell, endocytosis, and most importantly caveolae formation. As a consequence, there is… read more here.
Keywords: nmr spectroscopy; caveolin; preparation caveolin; spectroscopy ... See more keywords
Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7374-3_14
Abstract: Proton nuclear magnetic resonance spectroscopy (1H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of… read more here.
Keywords: nmr spectroscopy; analysis lipid; spectroscopy; lipid components ... See more keywords
Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7386-6_20
Abstract: This paper describes three protocols for identifying interacting surfaces on 15N-labeled target proteins of known structure by using in-cell NMR spectroscopy. The first protocol describes how to identify protein quinary structure interaction surfaces in prokaryotes… read more here.
Keywords: nmr spectroscopy; cell nmr; spectroscopy; using cell ... See more keywords
19F NMR Spectroscopy for the Analysis of DNA G-Quadruplex Structures Using 19F-Labeled Nucleobase.
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DOI: 10.1007/978-1-4939-9666-7_26
Abstract: G-quadruplex structures have been suggested to be biologically important in processes such as transcription and translation, gene expression and regulation in human cancer cells, and regulation of telomere length. Investigation of G-quadruplex structures associated with… read more here.
Keywords: spectroscopy analysis; nmr spectroscopy; quadruplex structures; spectroscopy ... See more keywords
Plant Metabolomics Using NMR Spectroscopy.
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DOI: 10.1007/978-1-4939-9690-2_19
Abstract: The major goal in plant metabolomics is to study complex extracts for the purposes of metabolic exploration and natural products discovery. To achieve this goal, plant metabolomics relies on accurate and selective acquisition of all possible… read more here.
Keywords: plant; plant metabolomics; using nmr; metabolomics using ... See more keywords