Isoelectric Focusing on Non-Denaturing Flatbed Gels.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8793-1_9
Abstract: Isoelectric focusing (IEF) serves as a very useful procedure for cell protein separation and characterization. We have used this method to study antibody clonotype changes. Here we discuss the use of a sensitive native flatbed… read more here.
Keywords: focusing non; non denaturing; denaturing flatbed; flatbed gels ... See more keywords
A simple method for non-denaturing purification of biotin-tagged proteins through competitive elution with free biotin.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2019-0088
Abstract: The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution conditions. Here, we use biotinylated bovine serum albumin as a working model… read more here.
Keywords: elution; biotin; non denaturing; biotin tagged ... See more keywords