Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases.
Sign Up to like & getrecommendations! Published in 2019 at "Current topics in microbiology and immunology"
DOI: 10.1007/82_2018_133
Abstract: Non-ribosomal peptide (NRP) natural products are one of the most promising resources for drug discovery and development because of their wide-ranging of therapeutic potential, and their behavior as virulence factors and signaling molecules. The NRPs… read more here.
Keywords: ribosomal peptide; based protein; nrps machinery; activity based ... See more keywords
Skyllamycins D and E, Non-Ribosomal Cyclic Depsipeptides from Lichen-Sourced Streptomyces anulatus.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of natural products"
DOI: 10.1021/acs.jnatprod.1c00547
Abstract: The skyllamycins are a class of heavily modified, non-ribosomal peptides, first isolated from Streptomyces sp. KY11784. A Streptomyces strain with potent antibiotic activity against Bacillus subtilis was isolated from a sample of the New Zealand… read more here.
Keywords: lichen sourced; cyclic depsipeptides; non ribosomal; depsipeptides lichen ... See more keywords
Recruitment and Regulation of the Non-ribosomal Peptide Synthetase Modifying Cytochrome P450 Involved in Nikkomycin Biosynthesis.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00081
Abstract: The β-hydroxylation of l-histidine is the first step in the biosynthesis of the imidazolone base of the antifungal drug nikkomycin. The cytochrome P450 (NikQ) hydroxylates the amino acid while it is appended via a phosphopantetheine… read more here.
Keywords: biosynthesis; ribosomal peptide; amino acid; peptide synthetase ... See more keywords
Simple and Rapid Non-ribosomal Peptide Synthetase Gene Assembly Using the SEAM-OGAB Method.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00565
Abstract: Recombination of biosynthetic gene clusters including those of non-ribosomal peptide synthetases (NRPSs) is essential for understanding the mechanisms of biosynthesis. Due to relatively huge gene cluster sizes ranging from 10 to 150 kb, the prevalence… read more here.
Keywords: gene assembly; seam ogab; non ribosomal; ribosomal peptide ... See more keywords
Streamlined Chemoenzymatic Synthesis of Cyclic Peptides by Non-ribosomal Peptide Cyclases.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c11082
Abstract: Macrocyclization improves the pharmaceutical properties of peptides; however, regio- and chemoselective intramolecular cyclizations remain challenging. Here we developed a streamlined chemoenzymatic approach to synthesize cyclic peptides by exploiting non-ribosomal peptide (NRP) cyclases. Linear peptides linked… read more here.
Keywords: cyclic peptides; non ribosomal; ribosomal peptide; chemoenzymatic synthesis ... See more keywords
Integrating genomics and metabolomics for scalable non-ribosomal peptide discovery
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23502-4
Abstract: Non-Ribosomal Peptides (NRPs) represent a biomedically important class of natural products that include a multitude of antibiotics and other clinically used drugs. NRPs are not directly encoded in the genome but are instead produced by… read more here.
Keywords: scalable non; integrating genomics; metabolomics scalable; non ribosomal ... See more keywords
Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-021-27512-0
Abstract: Non-Ribosomal Peptide Synthetases (NRPSs) assemble a diverse range of natural products with important applications in both medicine and agriculture. They consist of several multienzyme subunits that must interact with each other in a highly controlled… read more here.
Keywords: chain; domain; non ribosomal; type thioesterase ... See more keywords
Heterochiral coupling in non-ribosomal peptide macrolactamization
Sign Up to like & getrecommendations! Published in 2020 at "Nature Catalysis"
DOI: 10.1038/s41929-020-0456-7
Abstract: Heterochiral coupling is favoured in abiotic peptide bond formation, whereas biotic peptide bond formation is dominated by homochiral coupling. Here, we report that heterochiral coupling is a rather general paradigm in the head-to-tail macrolactamization of… read more here.
Keywords: macrolactamization; ribosomal peptide; non ribosomal; heterochiral coupling ... See more keywords
Exploring modular reengineering strategies to redesign the teicoplanin non-ribosomal peptide synthetase†
Sign Up to like & getrecommendations! Published in 2020 at "Chemical Science"
DOI: 10.1039/d0sc03483e
Abstract: Non-ribosomal peptide synthesis is an important biosynthesis pathway in secondary metabolism. In this study we have investigated modularisation and redesign strategies for the glycopeptide antibiotic teicoplanin. Using the relocation or exchange of domains within the… read more here.
Keywords: ribosomal peptide; strategies redesign; non ribosomal; modular reengineering ... See more keywords
Non-ribosomal peptide biosynthetic potential of the nematode symbiont Photorhabdus.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental microbiology reports"
DOI: 10.1111/1758-2229.13118
Abstract: Photorhabdus, the symbiotic bacteria of Heterorhabditis nematodes, has been reported to possess many non-ribosomal peptide synthetase (NRPS) biosynthesis gene clusters (BGCs). To provide an in-depth assessment of the non-ribosomal peptide biosynthetic potential of Photorhabdus, we… read more here.
Keywords: biosynthetic potential; photorhabdus; non ribosomal; ribosomal peptide ... See more keywords
Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0270359
Abstract: The growing demand for natural pigments in the industrial sector is a significant driving force in the development of production processes. The production of natural blue pigments, which have wide industrial applications, using microbial systems… read more here.
Keywords: platform; antioxidant activity; non ribosomal; ribosomal peptide ... See more keywords