An efficient cell‐free protein synthesis platform for producing proteins with pyrrolysine‐based noncanonical amino acids
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200096
Abstract: Incorporation of noncanonical amino acids (ncAAs) into proteins opens new opportunities in biotechnology and synthetic biology. Pyrrolysine (Pyl)‐based ncAAs are some of the most predominantly used, but expression systems suffer from low yields. Here, we… read more here.
Keywords: amino acids; platform; synthesis; noncanonical amino ... See more keywords
Engineering active lysostaphin variants that incorporate noncanonical amino acids and characterizing the effects of site‐specific PEGylation
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28360
Abstract: We describe a facile strategy to identify sites for the incorporation of noncanonical amino acids into lysostaphin—an enzyme that degrades the cell wall of Staphylococcus aureus—while retaining stapholytic activity. We used this strategy to generate… read more here.
Keywords: amino acids; lysostaphin variants; site specific; site ... See more keywords
Selection, Addiction and Catalysis: Emerging Trends for the Incorporation of Noncanonical Amino Acids into Peptides and Proteins in Vivo
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201800733
Abstract: Expanding the genetic code of organisms by incorporating noncanonical amino acids (ncAAs) into target proteins through the suppression of stop codons in vivo has profoundly impacted how we perform protein modification or detect proteins and… read more here.
Keywords: noncanonical amino; addiction; amino acids; catalysis ... See more keywords
Recent Applications of Carbon‐Nitrogen Lyases in Asymmetric Synthesis of Noncanonical Amino Acids and Heterocyclic Compounds
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000214
Abstract: Carbon‐nitrogen (C−N) lyases are enzymes that normally catalyze the cleavage of C−N bonds. Reversing this reaction towards carbon‐nitrogen bond formation can be a powerful approach to prepare valuable compounds that could find applications in everyday… read more here.
Keywords: nitrogen; noncanonical amino; amino acids; nitrogen lyases ... See more keywords
Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7574-7_8
Abstract: The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native… read more here.
Keywords: noncanonical amino; amino; amino acids; protein engineering ... See more keywords
A Bifunctional Noncanonical Amino Acid: Synthesis, Expression, and Residue-Specific Proteome-wide Incorporation.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00397
Abstract: Mapping of weak and hence transient interactions between low-abundance interacting molecules is still a major challenge in systems biology and protein biochemistry. Therefore, additional system-wide acting tools are needed to determine protein interactomics. Most important… read more here.
Keywords: biochemistry; noncanonical amino; amino acid; proteome wide ... See more keywords
Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons.
Sign Up to like & getrecommendations! Published in 2021 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00120
Abstract: We recently described an orthogonal initiator tRNA (itRNATy2) that can initiate protein synthesis with noncanonical amino acids (ncAAs) in response to the UAG nonsense codon. Here, we report that a mutant of itRNATy2 (itRNATy2AUA) can… read more here.
Keywords: noncanonical amino; initiator; amino acids; genetic encoding ... See more keywords
Evaluating Translational Efficiency of Noncanonical Amino Acids to Inform the Design of Druglike Peptide Libraries
Sign Up to like & getrecommendations! Published in 2023 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00712
Abstract: Advances in genetic code reprogramming have allowed the site-specific incorporation of noncanonical functionalities into polypeptides and proteins, providing access to wide swaths of chemical space via in vitro translation techniques like mRNA display. Prior efforts… read more here.
Keywords: amino acids; amino; amino acid; efficiency ... See more keywords
Identification of Macrocyclic Peptide Families from Combinatorial Libraries Containing Noncanonical Amino Acids Using Cheminformatics and Bioinformatics Inspired Clustering.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00159
Abstract: In the past decade, macrocyclic peptides gained increasing interest as a new therapeutic modality to tackle intracellular and extracellular therapeutic targets that had been previously classified as "undruggable". Several technological advances have made discovering macrocyclic… read more here.
Keywords: amino acids; identification; peptide; macrocyclic peptide ... See more keywords
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00604
Abstract: The UAG-based genetic code expansion (GCE) enables site-specific incorporation of noncanonical amino acids (ncAAs) harboring novel chemical functionalities in specific target proteins. However, most GCE studies were done in several whole-genome engineered chassis cells whose… read more here.
Keywords: amino acids; bacillus subtilis; expression; gce ... See more keywords
Enhancing Protein Stability with Genetically Encoded Noncanonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b07157
Abstract: The ability to add noncanonical amino acids to the genetic code may allow one to evolve proteins with new or enhanced properties using a larger set of building blocks. To this end, we have been… read more here.
Keywords: noncanonical amino; protein stability; enhancing protein; amino acids ... See more keywords