CRISPRi repression of nonhomologous endâjoining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26404
Abstract: In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most… read more here.
Keywords: homologous recombination; repression; nonhomologous end; crispri ... See more keywords
Deletions associated with stabilization of the Top1 cleavage complex in yeast are products of the nonhomologous end-joining pathway
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1914081116
Abstract: Significance The separation of DNA strands during replication and transcription creates torsional stress (supercoiling) that requires resolution by topoisomerases. Topoisomerase 1 (Top1) removes supercoils by transiently nicking one DNA strand, which allows its rotation around… read more here.
Keywords: end joining; stabilization; dna; cleavage ... See more keywords
Histone Variant macroH2A1.1 Enhances Nonhomologous End Joining-dependent DNA Double-strand-break Repair and Reprogramming Efficiency of Human iPSCs.
Sign Up to like & getrecommendations! Published in 2022 at "Stem cells"
DOI: 10.1093/stmcls/sxab004
Abstract: DNA damage repair (DDR) is a safeguard for genome integrity maintenance. Increasing DDR efficiency could increase the yield of induced pluripotent stem cells (iPSC) upon reprogramming from somatic cells. The epigenetic mechanisms governing DDR during… read more here.
Keywords: variant macroh2a1; macroh2a1; repair; macroh2a1 macroh2a1 ... See more keywords
Nonhomologous end-joining repair is likely involved in the repair of double-stranded DNA breaks induced by riluzole in melanoma cells.
Sign Up to like & getrecommendations! Published in 2019 at "Melanoma Research"
DOI: 10.1097/cmr.0000000000000652
Abstract: Our group described the oncogenic potential of a normal neuronal receptor, metabotropic glutamate receptor 1 (GRM1/mGluR1, gene/protein), when aberrantly expressed in melanocytes led to cell transformation in vitro and spontaneous metastatic tumors in vivo. Earlier,… read more here.
Keywords: repair; dna; nonhomologous end; end joining ... See more keywords
Meiotic DNA Repair in the Nucleolus Employs a Nonhomologous End-Joining Mechanism[OPEN]
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DOI: 10.1105/tpc.19.00367
Abstract: The rDNA is repaired via NHEJ during meiosis while being protected from canonical meiotic DSB formation and homologous recombination. Ribosomal RNA genes are arranged in large arrays with hundreds of rDNA units in tandem. These… read more here.
Keywords: end joining; meiosis; dna; nonhomologous end ... See more keywords
Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes
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DOI: 10.1128/mbio.01541-19
Abstract: Parasites tend to evolve small and compact genomes, generally endowed with a high mutation rate, compared with those of their free-living relatives. However, the mechanisms by which they achieve these features, independently in unrelated lineages,… read more here.
Keywords: nonhomologous end; loss; end joining; classical nonhomologous ... See more keywords
m6A-mediated nonhomologous end joining (NHEJ) pathway regulates senescence in Brachionus plicatilis (Rotifera).
Sign Up to like & getrecommendations! Published in 2023 at "Archives of gerontology and geriatrics"
DOI: 10.2139/ssrn.4333043
Abstract: Epigenetic modifications play an important role in the regulation of senescence. N6-methyladenosine (m6A) is the most abundant modification of mRNA. However, the impact of m6A on senescence remains largely unknown at the animal individual level.… read more here.
Keywords: end joining; joining nhej; nonhomologous end; nhej pathway ... See more keywords