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Published in 2017 at "Nature Protocols"
DOI: 10.1038/nprot.2016.160
Abstract: At present, several assays that use radioisotope labeling to quantify the degradation of long-lived proteins have been developed to measure autophagic flux. Here, we describe a nonradioactive pulse–chase protocol using L-azidohomoalanine (AHA) labeling to quantify…
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Keywords:
degradation;
nonradioactive quantification;
quantification autophagic;
protein degradation ... See more keywords