Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling
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DOI: 10.1038/nprot.2016.160
Abstract: At present, several assays that use radioisotope labeling to quantify the degradation of long-lived proteins have been developed to measure autophagic flux. Here, we describe a nonradioactive pulse–chase protocol using L-azidohomoalanine (AHA) labeling to quantify… read more here.
Keywords: degradation; nonradioactive quantification; quantification autophagic; protein degradation ... See more keywords