Media development and process parameter optimization using statistical experimental designs for the production of nonribosomal peptides in Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Electronic Journal of Biotechnology"
DOI: 10.1016/j.ejbt.2021.05.001
Abstract: Abstract Background Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and… read more here.
Keywords: media development; process; production; escherichia coli ... See more keywords
Incarnatapeptins A and B, Nonribosomal Peptides Discovered Using Genome Mining and 1H/15N HSQC-TOCSY.
Sign Up to like & getrecommendations! Published in 2020 at "Organic letters"
DOI: 10.1021/acs.orglett.0c00818
Abstract: Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an 15N NMR-based… read more here.
Keywords: nonribosomal peptides; incarnatapeptins nonribosomal; mining 15n; genome mining ... See more keywords
Bioinformatic Analysis Reveals both Oversampled and Underexplored Biosynthetic Diversity in Nonribosomal Peptides
Sign Up to like & getrecommendations! Published in 2023 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00761
Abstract: The traditional natural product discovery approach has accessed only a fraction of the chemical diversity in nature. The use of bioinformatic tools to interpret the instructions encoded in microbial biosynthetic genes has the potential to… read more here.
Keywords: prediction algorithms; biosynthetic diversity; bioinformatic analysis; diversity ... See more keywords
Free Piperazic Acid as a Precursor to Nonribosomal Peptides.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03660
Abstract: Piperazic acid (Piz) is a nonproteinogenic amino acid possessing a rare nitrogen-nitrogen bond. However, little is known about how Piz is incorporated into nonribosomal peptides, including whether adenylation domains specific to Piz exist. In this… read more here.
Keywords: free piperazic; piperazic acid; adenylation; nonribosomal peptides ... See more keywords
Discovery of cationic nonribosomal peptides as Gram-negative antibiotics through global genome mining
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DOI: 10.1038/s41467-018-05781-6
Abstract: The worldwide prevalence of infections caused by antibiotic-resistant Gram-negative bacteria poses a serious threat to public health due to the limited therapeutic alternatives. Cationic peptides represent a large family of antibiotics and have attracted interest… read more here.
Keywords: gram negative; gram; cationic nonribosomal; nonribosomal peptides ... See more keywords
Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Fungi"
DOI: 10.3390/jof7050374
Abstract: Previously, DNA microarrays analysis showed that, in co-culture with Bacillus subtilis, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of Aspergillus niger is downregulated. Based on phylogenetic and synteny analyses, we show here… read more here.
Keywords: peptides synthetase; gene cluster; nonribosomal peptides; biosynthetic gene ... See more keywords