Complement C1q in plasma induces nonspecific binding of poly(acrylic acid)-coated upconverting nanoparticle antibody conjugates
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04021-7
Abstract: Upconverting nanoparticles are attractive reporters for immunoassays, because their high specific activity and lack of autofluorescence background enable their detection at extremely low concentrations. However, the sensitivity achieved with heterogeneous sandwich immunoassays using nanoparticle reporters… read more here.
Keywords: nonspecific binding; poly acrylic; acrylic acid; acid coated ... See more keywords
Zwitterion and Oligo(Ethylene Glycol) Synergy Minimizes Nonspecific Binding of Compact Quantum Dots.
Sign Up to like & getrecommendations! Published in 2020 at "ACS nano"
DOI: 10.1021/acsnano.9b08658
Abstract: Quantum dots are a class of fluorescent nanocrystals in development as labels for molecular imaging in cells and tissues. Recently, coatings for quantum dots based on multidentate polymers have improved labeling performance in a range… read more here.
Keywords: oligo ethylene; fixed cells; nonspecific binding; zwitterion oligo ... See more keywords
Zwitterionand Oligo(ethylene glycol) Synergy MinimizesNonspecific Binding of Compact Quantum Dots
Sign Up to like & getrecommendations! Published in 2020 at "ACS Nano"
DOI: 10.1021/acsnano.9b08658.s001
Abstract: Quantum dots (QDs) are a class of fluorescent nanocrystals in development as labels for molecular imaging in cells and tissues. Recently, coatings for quantum dots based on multidentate polymers have improved labeling performance in a… read more here.
Keywords: oligo ethylene; fixed cells; nonspecific binding; ethylene glycol ... See more keywords
Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand–Analyte Interactions
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c05659
Abstract: Quantitative analysis of protein–protein interactions (PPIs) using biolayer interferometry (BLI) requires effective suppression of nonspecific binding (NSB) between analytes and biosensors. In particular, the study of weak interactions (i.e., KD > 1 μM) requires high… read more here.
Keywords: weak interactions; biolayer interferometry; new nsb; nonspecific binding ... See more keywords
Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-02420-x
Abstract: There is evidence that the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel is highly expressed at the apical pole of ciliated cells in human bronchial epithelium (HBE), however recent studies have detected little CFTR… read more here.
Keywords: epithelium; cftr; cells human; nonspecific binding ... See more keywords
Butylidenephthalide facilitates contractions via nonspecific binding to receptors in isolated guinea-pig vas deferens
Sign Up to like & getrecommendations! Published in 2019 at "Pharmaceutical Biology"
DOI: 10.1080/13880209.2019.1619781
Abstract: Abstract Context: Butylidenephthalide (Bdph) has been reported to inhibit rat uterine contractions, but significantly potentiate the noradrenaline (NA)-induced contractions in guinea-pig vas deferens (GPVDs). Objective: The present study elucidates the binding specificity of Bdph in… read more here.
Keywords: guinea pig; bdph; vas deferens; pig vas ... See more keywords
Effect of the nonspecific binding in differential impedance biosensing.
Sign Up to like & getrecommendations! Published in 2019 at "Biointerphases"
DOI: 10.1116/1.5082717
Abstract: The detection limits of impedance biosensors are dictated by the presence of background nonspecific binding, yet almost all the detection limits reported in the literature are determined using a clean buffer solution without confronting this… read more here.
Keywords: effect nonspecific; nonspecific binding; impedance; differential impedance ... See more keywords
Dielectrophoresis: An Approach to Increase Sensitivity, Reduce Response Time and to Suppress Nonspecific Binding in Biosensors?
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12100784
Abstract: The performance of receptor-based biosensors is often limited by either diffusion of the analyte causing unreasonable long assay times or a lack of specificity limiting the sensitivity due to the noise of nonspecific binding. Alternating… read more here.
Keywords: sensitivity; dielectrophoresis approach; biosensors dielectrophoresis; mass transfer ... See more keywords
A Bit Stickier, a Bit Slower, a Lot Stiffer: Specific vs. Nonspecific Binding of Gal4 to DNA
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22083813
Abstract: Transcription factors regulate gene activity by binding specific regions of genomic DNA thanks to a subtle interplay of specific and nonspecific interactions that is challenging to quantify. Here, we exploit Reflective Phantom Interface (RPI), a… read more here.
Keywords: gal4 dna; bit; binding gal4; nonspecific binding ... See more keywords
Bioanalysis of INCB000928 in hemodialysate: prevention of nonspecific binding and validation of surrogate matrices.
Sign Up to like & getrecommendations! Published in 2022 at "Bioanalysis"
DOI: 10.4155/bio-2022-0188
Abstract: Aim: To develop and validate a bioanalytical method for the quantification of INCB000928 in hemodialysate. Materials & methods: Blank dialysate and phosphate-buffered saline were compared with hemodialysate for surrogate matrix selection. Direct addition of internal… read more here.
Keywords: incb000928 hemodialysate; surrogate matrices; hemodialysate; nonspecific binding ... See more keywords