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Published in 2017 at "Fish Physiology and Biochemistry"
DOI: 10.1007/s10695-017-0439-z
Abstract: In ectotherms, temperature modulates oxidative stress, a key driver of aging. However, the effects of temperature on oxidative stress have not been investigated at several life stages of an ectotherm. In order to improve understanding…
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Keywords:
temperature;
fish acclimatized;
lipid peroxidation;
nothobranchius furzeri ... See more keywords
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Published in 2019 at "Aquatic toxicology"
DOI: 10.1016/j.aquatox.2019.05.009
Abstract: Although aquatic organisms are increasingly exposed to pollutants and abnormally high temperatures as a consequence of climate change, interactive effects between those stressors remain poorly assessed. Especially in ectotherms, such as fish, increases in ambient…
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Keywords:
interactive effects;
temperature;
killifish nothobranchius;
effects dca ... See more keywords
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Published in 2017 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2017.05.047
Abstract: Nothobranchius furzeri is a promising model for ecotoxicological research due to the species' short life cycle and the production of drought-resistant eggs. Although the species is an emerging vertebrate fish model for several fundamental as…
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Keywords:
chronic sensitivity;
copper;
sensitivity;
model ... See more keywords
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Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-65930-0
Abstract: Dietary alteration is one of the most universally effective aging interventions, making its standardization a fundamental need for model organisms in aging. In this dietetic study we address the current lack of standardized formulated diet…
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Keywords:
standardization;
killifish nothobranchius;
turquoise killifish;
nothobranchius furzeri ... See more keywords
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Published in 2020 at "Journal of the Royal Society Interface"
DOI: 10.1098/rsif.2020.0217
Abstract: Some species have a longer lifespan than others, but usually lifespan is correlated with typical body weight. Here, we study the lifetime evolution of the metabolic behaviour of Nothobranchius furzeri, a killifish with an extremely…
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Keywords:
comparative analysis;
analysis metabolic;
transcriptomic features;
analysis ... See more keywords
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Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107738
Abstract: The African turquoise killifish (Nothobranchius furzeri) is an extremely short-lived vertebrate that has emerged as a powerful model organism for several research areas, including aging and embryonic diapause, which is the temporary suspension of embryonic…
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Keywords:
killifish nothobranchius;
african turquoise;
nothobranchius furzeri;
turquoise killifish ... See more keywords
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Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107739
Abstract: Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates…
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Keywords:
furzeri nothobranchiidae;
practices histological;
nothobranchius furzeri;
good practices ... See more keywords
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Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107745
Abstract: Blood withdrawal is a common procedure performed on laboratory animals to monitor key processes and indicators of fish health and physiology, such as hematopoiesis, hemostasis, and lipid and glucose metabolism. Moreover, the ability to extract…
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Keywords:
blood sampling;
nonlethal blood;
sampling killifish;
nothobranchius furzeri ... See more keywords
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Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107748
Abstract: Transposable elements (TEs) comprise large fractions of eukaryotic genomes, but their repetitive nature and high copy number makes bioinformatic analyses more complex. Here, we report three robust pipelines to analyze TE expression from RNA-seq data…
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Keywords:
seq data;
transposable elements;
rna seq;
differential expression ... See more keywords
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Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107752
Abstract: The African turquoise killifish Nothobranchius furzeri is an emerging research organism known for its short life span and long-term diapause. Diapause is a unique dormant state that suspends embryonic development without tradeoffs in the adulthood…
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Keywords:
diapause;
turquoise killifish;
furzeri;
african turquoise ... See more keywords
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Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107790
Abstract: Immunofluorescence is a widely used technique to visualize the localization of proteins of interest. Nucleoside analogs, such as 5-ethynyl-2'-deoxyuridine (EdU), are incorporated into newly synthesized DNA and enable permanent labeling of newly divided cells. Both…
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Keywords:
edu immunofluorescence;
immunofluorescence staining;
nothobranchius furzeri;
furzeri organotypic ... See more keywords