The use of clade‐specific PCR assays to identify novel nitrilase genes from environmental isolates
Sign Up to like & getrecommendations! Published in 2019 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.700
Abstract: Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio‐pharmaceuticals and industrial applications facilitates the move towards… read more here.
Keywords: novel nitrilase; nitrilase; clade specific; pcr ... See more keywords
A Triple-Focus Workflow of a Novel Nitrilase for Simultaneously Optimizing Activity, Thermostability, and Specificity.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.5c07983
Abstract: Nitrilases provide a sustainable route for directly converting nitriles to carboxylic acids. However, efficient biocatalytic synthesis of the agrochemical intermediate 3,6-dichloropicolinic acid(3,6-DCPA) from 3,6-dichloropicolinonitrile (3,6-DCPN) remains unreported. We identified a novel nitrilase from Rhodococcus pyridinivorans… read more here.
Keywords: novel nitrilase; thermostability specificity; activity thermostability; activity ... See more keywords