Cytosolic expression, solution structures, and molecular dynamics simulation of genetically encodable disulfide‐rich de novo designed peptides
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3453
Abstract: Disulfide‐rich peptides represent an important protein family with broad pharmacological potential. Recent advances in computational methods have made it possible to design new peptides which adopt a stable conformation de novo. Here, we describe a… read more here.
Keywords: novo designed; disulfide rich; solution; rich novo ... See more keywords
Towards functional de novo designed proteins.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2019.06.011
Abstract: Our ability to design completely de novo proteins is improving rapidly. This is true of all three main approaches to de novo protein design, which we define as: minimal, rational and computational design. Together, these… read more here.
Keywords: designed proteins; novo designed; towards functional; functional novo ... See more keywords
New Class of Drug Modalities: Directed Evolution of a De Novo Designed Helix-Loop-Helix Peptide to Bind VEGF for Tumor Growth Inhibition.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00940
Abstract: As a small affinity molecule to serve as an alternative to antibodies, we have developed a conformationally constrained peptide with a de novo designed helix-loop-helix (HLH) scaffold. To evaluate its potential for biomedical applications, we… read more here.
Keywords: novo designed; loop helix; designed helix; growth ... See more keywords
De Novo-Designed Near-Infrared Nanoaggregates for Super-Resolution Monitoring of Lysosomes in Cells, in Whole Organoids and In Vivo.
Sign Up to like & getrecommendations! Published in 2019 at "ACS nano"
DOI: 10.1021/acsnano.9b08011
Abstract: As the cleaners of cells, lysosomes play an important role in circulating organic matter within cells, recovering damaged organelles, and removing waste via endocytosis. Because lysosome dysfunction is associated with various diseases-lysosomal storage diseases, inherited… read more here.
Keywords: resolution; resolution imaging; novo designed; near infrared ... See more keywords
De Novo-Designed α-Helical Barrels as Receptors for Small Molecules.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00225
Abstract: We describe de novo-designed α-helical barrels (αHBs) that bind and discriminate between lipophilic biologically active molecules. αHBs have five or more α-helices arranged around central hydrophobic channels the diameters of which scale with oligomer state.… read more here.
Keywords: barrels receptors; receptors small; novo designed; small molecules ... See more keywords
De-Novo-Designed Translation-Repressing Riboregulators for Multi-Input Cellular Logic
Sign Up to like & getrecommendations! Published in 2019 at "Nature chemical biology"
DOI: 10.1038/s41589-019-0388-1
Abstract: Efforts to construct synthetic biological circuits with more complex functions have often been hindered by the idiosyncratic behavior, limited dynamic range, and crosstalk of commonly utilized parts. Here, we employ de novo RNA design to… read more here.
Keywords: designed translation; translation repressing; novo designed; repressing riboregulators ... See more keywords
Designed for life: biocompatible de novo designed proteins and components
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the Royal Society Interface"
DOI: 10.1098/rsif.2018.0472
Abstract: A principal goal of synthetic biology is the de novo design or redesign of biomolecular components. In addition to revealing fundamentally important information regarding natural biomolecular engineering and biochemistry, functional building blocks will ultimately be… read more here.
Keywords: designed proteins; novo designed; designed life; proteins components ... See more keywords
De novo-designed transmembrane domains tune engineered receptor functions
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.75660
Abstract: De novo-designed receptor transmembrane domains (TMDs) present opportunities for precise control of cellular receptor functions. We developed a de novo design strategy for generating programmed membrane proteins (proMPs): single-pass α-helical TMDs that self-assemble through computationally… read more here.
Keywords: receptor functions; novo designed; receptor; transmembrane domains ... See more keywords