Facile de Novo Sequencing of Tetrazine‐Cyclized Peptides through UV‐Induced Ring‐Opening and Cleavage from the Solid Phase
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200590
Abstract: While most FDA‐approved peptide drugs are cyclic, the robust cyclization chemistry of peptides and the deconvolution of cyclic peptide sequences by using tandem mass spectrometry render cyclic peptide drug discovery difficult. Here we present the… read more here.
Keywords: sequencing tetrazine; cyclic peptides; novo sequencing; solid phase ... See more keywords
NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2016.12.003
Abstract: A simple and effective de novo sequencing strategy assisted by non-isobaric peptide termini labeling, NIPTL-Novo, was established. The y-series ions and b-series ions of peptides can be clearly distinguished according to the different mass tags… read more here.
Keywords: isobaric peptide; peptide; non isobaric; termini labeling ... See more keywords
De novo sequencing and initial annotation of the Mongolian gerbil (Meriones unguiculatus) genome.
Sign Up to like & getrecommendations! Published in 2019 at "Genomics"
DOI: 10.1016/j.ygeno.2018.03.001
Abstract: The Mongolian gerbil (Meriones unguiculatus) is a member of the rodent family that displays several features not found in mice or rats, including sensory specializations and social patterns more similar to those in humans. These… read more here.
Keywords: novo sequencing; gerbil; gerbil meriones; sequencing initial ... See more keywords
Dissociation Pattern of Sodiated Amide Peptides as a Tool for De Novo Sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.0c00269
Abstract: As part of the de novo sequencing issue, new approaches have to be found to sequence small natural peptides ( read more here.
Keywords: sodiated amide; novo sequencing; dissociation pattern; pattern sodiated ... See more keywords
De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2213432119
Abstract: Significance Cytochrome c (cyt c) is an abundant heme protein that, in its native conformation, plays essential roles in mitochondrial bioenergetics and apoptotic signaling in mammalian cells. The dynamic properties of cyt c confers substantial… read more here.
Keywords: alternative conformations; construction unique; novo sequencing; unique antibody ... See more keywords
De novo sequencing of the transcriptome reveals regulators of the floral transition in Fargesia macclureana (Poaceae)
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6418-2
Abstract: BackgroundFargesia macclureana (Poaceae) is a woody bamboo species found on the Qinghai–Tibet Plateau (QTP) approximately 2000 ~ 3800 m above sea level. It rarely blossoms in the QTP, but it flowered 20 days after growing in our… read more here.
Keywords: transcriptome reveals; sequencing transcriptome; macclureana; macclureana poaceae ... See more keywords
De novo sequencing of Bletilla striata (Orchidaceae) transcriptome and identification of genes involved in polysaccharide biosynthesis
Sign Up to like & getrecommendations! Published in 2020 at "Genetics and Molecular Biology"
DOI: 10.1590/1678-4685-gmb-2019-0417
Abstract: Abstract Bletilla striata polysaccharide (BSP) is the main component of Bletilla striata, which has important pharmacological and pharmacological effects; however, due to the lack of genetic data, the metabolic pathways of BSP remain unclear. For… read more here.
Keywords: genes involved; bletilla striata; novo sequencing; biosynthesis ... See more keywords
De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2021.652957
Abstract: Vibrio parahaemolyticus is a common pathogenic marine bacterium that causes gastrointestinal infections and other health complications, which could be life-threatening to immunocompromised patients. For the past two decades, the pathogenicity of environmental V. parahaemolyticus has… read more here.
Keywords: vibrio parahaemolyticus; environmental parahaemolyticus; pathogenicity; parahaemolyticus ... See more keywords
Biological Activity of Cyclic Peptide Extracted from Sphaeranthus amaranthoides Using De Novo Sequencing Strategy by Mass Spectrometry for Cancer
Sign Up to like & getrecommendations! Published in 2023 at "Biology"
DOI: 10.3390/biology12030412
Abstract: Simple Summary Cancer therapy today has benefited from multifaceted approaches in early detection and diagnosis, but weak prognosis still hinders progress, as it is a barricade for guaranteed successful treatment. The present study checks the… read more here.
Keywords: mass spectrometry; novo sequencing; cyclic peptide; sphaeranthus amaranthoides ... See more keywords
The Current State-of-the-Art Identification of Unknown Proteins Using Mass Spectrometry Exemplified on De Novo Sequencing of a Venom Protease from Bothrops moojeni
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27154976
Abstract: (1) Background: The amino acid sequence elucidation of peptides from the gas phase fragmentation mass spectra, de novo sequencing, is a valuable method for the identification of unknown proteins complementary to Edman sequencing. It is… read more here.
Keywords: identification unknown; novo sequencing; amino acid; unknown proteins ... See more keywords