Does the nuclear envelope retain its identity during mitosis?
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14568
Abstract: During mitosis in metazoan species, the nuclear envelope (NE) undergoes breakdown, and its fragments are absorbed within the membranous network of the endoplasmic reticulum (ER). Past observations by fluorescence microscopy led researchers to think that… read more here.
Keywords: mitosis; identity mitosis; nuclear envelope; identity ... See more keywords
Downregulation of YAP Activity Restricts P53 Hyperactivation to Promote Cell Survival in Confinement.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202302228
Abstract: Cell migration through confining three dimensional (3D) topographies can lead to loss of nuclear envelope integrity, DNA damage, and genomic instability. Despite these detrimental phenomena, cells transiently exposed to confinement do not usually die. Whether… read more here.
Keywords: nuclear envelope; cell; yap activity; confinement ... See more keywords
Measurement of Arabidopsis thaliana Nuclear Size and Shape.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0179-2_8
Abstract: Gene expression is tightly linked to the position of genes in the nucleus. Genomic regions associated with the nuclear envelope are usually repressed, including the heterochromatin carrying chromocenters. The shape and size of nuclei varies… read more here.
Keywords: nuclear envelope; measurement arabidopsis; size; arabidopsis thaliana ... See more keywords
Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_1
Abstract: The nuclear envelope (NE), a double membrane that separates nuclear components from the cytoplasm, undergoes a breakdown and reformation during cell division. To trace NE dynamics, the NE needs to be labeled with a fluorescent… read more here.
Keywords: nuclear envelope; nuclear membrane; inner nuclear; fluorescent labeling ... See more keywords
Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_18
Abstract: We describe a method for rapid identification of protein kinase substrates within the nuclear envelope. Open mitosis in higher eukaryotes is characterized by nuclear envelope breakdown (NEBD) concerted with disassembly of the nuclear lamina and… read more here.
Keywords: kinase; kinase targeted; purification cdk; nuclear envelope ... See more keywords
Loss of Nuclear Envelope Integrity in Aging and Disease.
Sign Up to like & getrecommendations! Published in 2018 at "International review of cell and molecular biology"
DOI: 10.1016/bs.ircmb.2017.07.013
Abstract: The nuclear envelope (NE) serves as a central organizing unit for the eukaryotic cell. By virtue of its highly selective, semipermeable barrier function, the NE shields the enclosed genetic material, while at the same time… read more here.
Keywords: nuclear envelope; integrity; aging disease; loss ... See more keywords
Quantitative Brightness Analysis of Protein Oligomerization in the Nuclear Envelope.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.05.044
Abstract: Brightness analysis of fluorescence fluctuation experiments has been used to successfully measure the oligomeric state of proteins at the plasma membrane, in the nucleoplasm, and in the cytoplasm of living cells. Here we extend brightness… read more here.
Keywords: nuclear envelope; analysis; membrane; brightness analysis ... See more keywords
The Dynamic Nature of the Nuclear Envelope
Sign Up to like & getrecommendations! Published in 2018 at "Current Biology"
DOI: 10.1016/j.cub.2018.01.073
Abstract: Eukaryotes characteristically organize their genome in a separate compartment, the nucleus, which is surrounded by the nuclear envelope as a barrier. Ruptures of the nuclear envelope and exposure of chromatin threaten cell viability and cause… read more here.
Keywords: microscopy; nuclear envelope; dynamic nature; envelope ... See more keywords
Active Fluctuations of the Nuclear Envelope Shape the Transcriptional Dynamics in Oocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Developmental cell"
DOI: 10.1016/j.devcel.2019.09.010
Abstract: Nucleus position in cells can act as a developmental cue. Mammalian oocytes position their nucleus centrally using an F-actin-mediated pressure gradient. The biological significance of nucleus centering in mammalian oocytes being unknown, we sought to… read more here.
Keywords: nuclear envelope; actin; pressure gradient; fluctuations nuclear ... See more keywords
Mechanical LINCs of the nuclear envelope: Where SUN meets KASH
Sign Up to like & getrecommendations! Published in 2018 at "Extreme Mechanics Letters"
DOI: 10.1016/j.eml.2018.01.010
Abstract: Author(s): Jahed, Z; Mofrad, MRK | Abstract: © 2018 Elsevier Ltd The transmission of mechanical signals across the nuclear envelope is primarily mediated by linkers of the nucleoskeleton and cytoskeleton (LINC complexes). These complexes bridge… read more here.
Keywords: linc complexes; nuclear envelope; envelope; lincs nuclear ... See more keywords
The nuclear envelope: target and mediator of the apoptotic process.
Sign Up to like & getrecommendations! Published in 2020 at "Cell death discovery"
DOI: 10.1038/s41420-020-0256-5
Abstract: Apoptosis is characterized by the destruction of essential cell organelles, including the cell nucleus. The nuclear envelope (NE) separates the nuclear interior from the cytosol. During apoptosis, the apoptotic machinery, in particular caspases, increases NE… read more here.
Keywords: death; mediator; nuclear envelope; apoptotic process ... See more keywords