Unraveling docking and initiation of mRNA export through the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200027
Abstract: The nuclear export of mRNA through the nuclear pore complex (NPC) is a process required for the healthy functioning of human cells, making it a critical area of research. However, the geometries of mRNA and… read more here.
Keywords: nuclear pore; pore complex; nuclear basket; microscopy ... See more keywords
Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_1
Abstract: Studying protein complexes in vitro requires the production of a relatively pure sample that maintains the full complement, native organization, and function of that complex. This can be particularly challenging to achieve for large, multi-component,… read more here.
Keywords: pore complexes; nuclear pore; affinity; cerevisiae nuclear ... See more keywords
Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast, and Plants.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_27
Abstract: Scanning electron microscopy (SEM) can be used to image nuclear pore complex (NPC) surface structure of from a number of organisms and model systems. With a field emission SEM , this is a medium resolution… read more here.
Keywords: nuclear pore; microscopy; microscopy sem; sem ... See more keywords
Nuclear Pore Complex Assembly Using Xenopus Egg Extract.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_3
Abstract: Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and… read more here.
Keywords: xenopus egg; nuclear pore; egg extract; pore complex ... See more keywords
Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_4
Abstract: In eukaryotic cells the nuclear envelope encloses the genome separating it from the rest of the cell. Nuclear pore complexes are large multi protein channels that perforate the nuclear envelope, connecting the nucleus and the… read more here.
Keywords: nuclear pore; nuclei using; permeability; isolated nuclei ... See more keywords
Polymer translocation through a hairy channel mimicking the inner plug of a nuclear pore complex
Sign Up to like & getrecommendations! Published in 2019 at "European Biophysics Journal"
DOI: 10.1007/s00249-019-01356-5
Abstract: A microscopic transport model of a polymer translocating through a nuclear pore complex (NPC) is presented based on self-consistent field theory (SCFT), with the NPC and its nucleoporins mimicked by a hairy channel. Multiple cell… read more here.
Keywords: polymer; pore complex; effect; translocation ... See more keywords
Nuclear pore complexes and regulation of gene expression.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2016.12.006
Abstract: Nuclear pore complexes (NPCs), are large multiprotein channels that penetrate the nuclear envelope connecting the nucleus to the cytoplasm. Accumulating evidence shows that besides their main role in regulating the exchange of molecules between these… read more here.
Keywords: pore complexes; gene expression; nuclear pore; gene ... See more keywords
Poring over chromosomes: mitotic nuclear pore complex segregation.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2019.01.002
Abstract: Eukaryotic cells rely on flux of macromolecules between the nucleus and the cytoplasm for growth and survival. Bidirectional transport is achieved through Nuclear Pore Complexes (NPCs) embedded in the Nuclear Envelope (NE). NPC proteins perform… read more here.
Keywords: mitotic nuclear; poring chromosomes; nuclear pore; segregation ... See more keywords
Relocation of Collapsed Forks to the Nuclear Pore Complex Depends on Sumoylation of DNA Repair Proteins and Permits Rad51 Association.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107635
Abstract: Expanded CAG repeats form stem-loop secondary structures that lead to fork stalling and collapse. Previous work has shown that these collapsed forks relocalize to nuclear pore complexes (NPCs) in late S phase in a manner… read more here.
Keywords: sumoylation; dna repair; fork; repair proteins ... See more keywords
Sol-gel transition in solutions of FG-Nups of the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2018 at "Extreme Mechanics Letters"
DOI: 10.1016/j.eml.2018.04.006
Abstract: The nuclear pore complex (NPC) is a giant molecular gate that regulates the transport of molecules and macromolecules across the nuclear envelop in a selective and directional manner. Using a coarse-grained one-bead-per-amino-acid model and percolation… read more here.
Keywords: solutions nups; pore complex; sol gel; nuclear pore ... See more keywords
Nuclear Pore Complexes Are Key Regulators of Oligodendrocyte Differentiation and Function
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DOI: 10.1016/j.neuron.2019.04.025
Abstract: Increasing evidence points to nuclear pore complexes as important regulators of cell fate and tissue homeostasis. A recent report by Liu et al. (2019) in this issue of Neuron uncovers that nucleoporin Seh1 is required for… read more here.
Keywords: complexes key; pore complexes; oligodendrocyte differentiation; nuclear pore ... See more keywords