Quantitative 3D structured illumination microscopy of nuclear structures
Sign Up to like & getrecommendations! Published in 2017 at "Nature Protocols"
DOI: 10.1038/nprot.2017.020
Abstract: 3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices,… read more here.
Keywords: image; microscopy; illumination microscopy; structured illumination ... See more keywords
Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-05963-2
Abstract: Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation… read more here.
Keywords: resolution; microscopy; super resolution; imaging nuclear ... See more keywords
3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers
Sign Up to like & getrecommendations! Published in 2022 at "Scientific Reports"
DOI: 10.1038/s41598-021-04443-w
Abstract: Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes of… read more here.
Keywords: microscopy; sub nuclear; nuclear structures; afm ... See more keywords
Benefits of Seismic Isolation for Nuclear Structures Subjected to Severe Earthquake
Sign Up to like & getrecommendations! Published in 2018 at "Science and Technology of Nuclear Installations"
DOI: 10.1155/2018/8017394
Abstract: The Fukushima accident has reiterated that the seismic safety is a clear necessity in the design of nuclear power plants. To overcome the weaknesses of the plant design, appropriate measures or interventions have thus to… read more here.
Keywords: benefits seismic; isolation nuclear; nuclear structures; isolation ... See more keywords