Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2022 at "Drug testing and analysis"
DOI: 10.1002/dta.3267
Abstract: Gene editing and subsequent cloning techniques offer great potential not only in genetic disease correction in domestic animals, but also in livestock production by enhancement of desirable traits. The existence of the technology, however, leaves… read more here.
Keywords: mitochondrial sequencing; nuclear transfer; cell nuclear; somatic cell ... See more keywords
State of the art of nuclear transfer technologies for assisting mammalian reproduction
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23615
Abstract: The transfer of nuclear genomic DNA from a cell to a previously enucleated oocyte or zygote constitutes one of the main tools for studying epigenetic reprogramming, nucleus–cytoplasm compatibility, pluripotency state, and for genetic preservation or… read more here.
Keywords: transfer; nuclear transfer; art nuclear; state art ... See more keywords
Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/7651_2018_118
Abstract: Stem cells have the potential to differentiate into specialized cell types under specific conditions in vivo or in vitro, which are used to cure many diseases related to aging. Somatic cell nuclear transfer (SCNT) can… read more here.
Keywords: nuclear transfer; aged cells; stem; stem cells ... See more keywords
Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9009-2_22
Abstract: Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can… read more here.
Keywords: somatic cell; cell nuclear; nuclear transfer; biology ... See more keywords
Episomal minicircles persist in periods of transcriptional inactivity and can be transmitted through somatic cell nuclear transfer into bovine embryos
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology Reports"
DOI: 10.1007/s11033-019-04624-x
Abstract: Episomal plasmids based on a scaffold/matrix attachment region (S/MAR) are extrachromosomal DNA entities that replicate once per cell cycle and are stably maintained in cells or tissue. We generated minicircles, episomal plasmids devoid of bacterial… read more here.
Keywords: somatic cell; cell nuclear; bovine; episomal minicircles ... See more keywords
55. EFFICACY OF NUCLEAR TRANSFER TO PREVENT THE INHERITED MITOCHONDRIAL PATHOLOGY
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive BioMedicine Online"
DOI: 10.1016/j.rbmo.2019.04.108
Abstract: Introduction Mitochondrial pathology is characterized by maternal inheritance. Women may be hidden mutation carriers, but in some cases the risk for descendants may reach up to 100%. Due to the uneven distribution of mitochondria during… read more here.
Keywords: nuclear transfer; pathology; transfer; mitochondrial pathology ... See more keywords
Genomic instability during reprogramming by nuclear transfer is DNA replication dependent
Sign Up to like & getrecommendations! Published in 2017 at "Nature Cell Biology"
DOI: 10.1038/ncb3485
Abstract: Somatic cells can be reprogrammed to a pluripotent state by nuclear transfer into oocytes, yet developmental arrest often occurs. While incomplete transcriptional reprogramming is known to cause developmental failure, reprogramming also involves concurrent changes in… read more here.
Keywords: nuclear transfer; dna replication; instability;
Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning
Sign Up to like & getrecommendations! Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1814514115
Abstract: Significance To investigate the epigenetic mechanism of pregnancy failure in mammals, we exploited the high rate of fetal loss in pig induced pluripotent stem cell (iPSC) nuclear transfer. We generated methylomes of pig iPSCs and… read more here.
Keywords: pig; pregnancy; cause; nuclear transfer ... See more keywords
Effects of Recloning on the Telomere Lengths of Mouse Terc+/- Nuclear Transfer-Derived Embryonic Stem Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Stem cells and development"
DOI: 10.1089/scd.2022.0115
Abstract: Haploinsufficiency of genes that participate in the telomere elongation and maintenance processes, such as Terc and Tert, often lead to premature aging related diseases such as dyskeratosis congenita and aplastic anemia. Previously we reported that… read more here.
Keywords: embryonic stem; round scnt; nuclear transfer; mouse terc ... See more keywords
Risk of pathogenic virus transmission by somatic cell nuclear transfer: implications for xenotransplantation
Sign Up to like & getrecommendations! Published in 2022 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioac120
Abstract: Abstract Using somatic cell nuclear transfer for the generation of cloned and transgenic animals bears the risk of transmission of viruses, either by the oocyte or by the introduced donor cell. There is evidence that… read more here.
Keywords: cell; nuclear transfer; transmission; somatic cell ... See more keywords
Comparative analysis of different nuclear transfer techniques to prevent the transmission of mitochondrial DNA variants.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular human reproduction"
DOI: 10.1093/molehr/gaz062
Abstract: Prevention of mitochondrial DNA (mtDNA) diseases may currently be possible using germline nuclear transfer (NT). However, scientific evidence to compare efficiency of different NT techniques to overcome mtDNA diseases is lacking. Here, we performed four… read more here.
Keywords: pb2t; mtdna; nuclear transfer; transfer ... See more keywords