Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-Associated Proteins in Escherichia coli.
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DOI: 10.1007/978-1-4939-8675-0_8
Abstract: Bacterial nucleoid-associated proteins are important in nucleoid-structuring, homeostasis of DNA supercoiling, and in regulation of transcription. Vice versa, transcription influences DNA supercoiling and possibly DNA-binding of nucleoid-associated proteins. Here, I describe genetic tools to study… read more here.
Keywords: study interplay; associated proteins; interplay transcription; nucleoid associated ... See more keywords
NapA (Rv0430), a Novel Nucleoid-Associated Protein that Regulates a Virulence Operon in Mycobacterium tuberculosis in a Supercoiling-Dependent Manner.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2019.02.029
Abstract: Comparison of Mycobacterium tuberculosis with Escherichia coli reveals a reduction in the diversity of DNA-managing proteins, such as DNA topoisomerases, although genome sizes are similar for the two species. The same is true for nucleoid-associated… read more here.
Keywords: rv0430 novel; virulence; tuberculosis; nucleoid associated ... See more keywords
Chromosome organization in bacteria: mechanistic insights into genome structure and function
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DOI: 10.1038/s41576-019-0185-4
Abstract: Bacterial chromosomes are folded to compact DNA and facilitate cellular processes. Studying model bacteria has revealed aspects of chromosome folding that are applicable to many species. Primarily controlled by nucleoid-associated proteins, chromosome folding is hierarchical,… read more here.
Keywords: associated proteins; chromosome folding; chromosome structure; nucleoid associated ... See more keywords
Bacteriophage protein Gp46 is a cross-species inhibitor of nucleoid-associated HU proteins
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2116278119
Abstract: Significance Histone-like protein from Escherichia coli strain U93 (HU) protein is the most abundant nucleoid-associated protein in bacteria, which plays a fundamental role in chromosomal compaction and organization. It is essential for most bacteria as… read more here.
Keywords: protein gp46; cross species; gp46 cross; protein ... See more keywords
A phage-encoded nucleoid associated protein compacts both host and phage DNA and derepresses H-NS silencing.
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DOI: 10.1093/nar/gkab678
Abstract: Nucleoid Associated Proteins (NAPs) organize the bacterial chromosome within the nucleoid. The interaction of the NAP H-NS with DNA also represses specific host and xenogeneic genes. Previously, we showed that the bacteriophage T4 early protein… read more here.
Keywords: microscopy; motb; dna; phage ... See more keywords
HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic acids research"
DOI: 10.1093/nar/gkab993
Abstract: The interplay between three-dimensional chromosome organisation and genomic processes such as replication and transcription necessitates in vivo studies of chromosome dynamics. Fluorescent organic dyes are often used for chromosome labelling in vivo. The mode of… read more here.
Keywords: associated protein; dna; genetically encoded; bacterial nucleoid ... See more keywords
Xenogeneic nucleoid-associated EnrR thwarts H-NS silencing of bacterial virulence with unique DNA binding
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DOI: 10.1093/nar/gkac180
Abstract: Abstract Type III and type VI secretion systems (T3/T6SS) are encoded in horizontally acquired genomic islands (GIs) that play crucial roles in evolution and virulence in bacterial pathogens. T3/T6SS expression is subjected to tight control… read more here.
Keywords: dna; nucleoid associated; dna binding; xenogeneic nucleoid ... See more keywords
Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif
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DOI: 10.1093/nar/gky257
Abstract: Abstract ParA and ParB homologs are involved in accurate chromosome segregation in bacteria. ParBs participate in the separation of ori domains by binding to parS palindromes, mainly localized close to oriC. In Pseudomonas aeruginosa neither… read more here.
Keywords: protein; associated protein; pseudomonas aeruginosa; nucleoid associated ... See more keywords
Direct inhibition of transcription in vitro by the isolated N-terminal domain of the Escherichia coli nucleoid-associated protein H-NS and by its paralogue Hha.
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DOI: 10.1099/mic.0.000780
Abstract: H-NS is an abundant nucleoid-associated protein in the enterobacteria that mediates both chromatin compaction and transcriptional silencing of numerous genes, especially those that have been acquired by horizontal transfer or that are involved in virulence… read more here.
Keywords: associated protein; transcription; terminal domain; nucleoid associated ... See more keywords
Unraveling novel roles of the Mycobacterium tuberculosis transcription factor Rv0081 in regulation of the nucleoidâassociated proteins Lsr2 and EspR, cholesterol utilization, and subversion of lysosomal trafficking in macrophages
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DOI: 10.1111/mmi.14895
Abstract: The transcriptional network of Mycobacterium tuberculosis is designed to enable the organism to withstand hostâassociated stresses and to exploit the host milieu for its own survival and multiplication. Rv0081 (MT0088) is a transcriptional regulator whose… read more here.
Keywords: mycobacterium tuberculosis; lysosomal trafficking; tuberculosis; nucleoid associated ... See more keywords
Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin
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DOI: 10.3389/fmicb.2023.1146406
Abstract: Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration… read more here.
Keywords: highly dynamic; integration host; nucleoid associated; mycobacterial ihf ... See more keywords