General Principles for Yield Optimization of Nucleoside Phosphorylase‐Catalyzed Transglycosylations†
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201900740
Abstract: The biocatalytic synthesis of natural and modified nucleosides with nucleoside phosphorylases offers the protecting‐group‐free direct glycosylation of free nucleobases in transglycosylation reactions. This contribution presents guiding principles for nucleoside phosphorylase‐mediated transglycosylations alongside mathematical tools for… read more here.
Keywords: optimization nucleoside; general principles; yield optimization; principles yield ... See more keywords
The Peculiar Case of the Hyper‐thermostable Pyrimidine Nucleoside Phosphorylase from Thermus thermophilus **
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DOI: 10.1002/cbic.202000679
Abstract: The poor solubility of many nucleosides and nucleobases in aqueous solution demands harsh reaction conditions (base, heat, cosolvent) in nucleoside phosphorylase‐catalyzed processes to facilitate substrate loading beyond the low millimolar range. This, in turn, requires… read more here.
Keywords: pyrimidine; thermus thermophilus; pyrimidine nucleoside; phosphorylase thermus ... See more keywords
Synthesis of Adenine Nucleosides by Transglycosylation using Two Sequential Nucleoside Phosphorylase‐Based Bioreactors with On‐Line Reaction Monitoring by using HPLC
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DOI: 10.1002/cctc.201701222
Abstract: Uridine phosphorylase from Clostridium perfringens (CpUP, EC 2.4.2.3) was immobilized covalently in an aminopropylsilica monolithic column (25 mm×4.6 mm) upon functionalization with glutaraldehyde. Imino bonds that result from the reaction between the enzyme and the support were… read more here.
Keywords: line reaction; transglycosylation; phosphorylase; synthesis ... See more keywords
Discovery of a Novel Inhibitor of Human Purine Nucleoside Phosphorylase by a Simple Hydrophilic Interaction Liquid Chromatography Enzymatic Assay
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DOI: 10.1002/cmdc.202000874
Abstract: Human purine nucleoside phosphorylase (HsPNP) belongs to the purine salvage pathway of nucleic acids. Genetic deficiency of this enzyme triggers apoptosis of activated T‐cells due to the accumulation of deoxyguanosine triphosphate (dGTP). Therefore, potential chemotherapeutic… read more here.
Keywords: purine nucleoside; human purine; discovery novel; interaction ... See more keywords
A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2423-1
Abstract: ObjectiveTo isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.ResultsBbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP… read more here.
Keywords: borstelensis lk01; brevibacillus borstelensis; pyrimidine nucleoside; bbpynp ... See more keywords
pH-Independent Heat Capacity Changes during Phosphorolysis Catalyzed by the Pyrimidine Nucleoside Phosphorylase from Geobacillus thermoglucosidasius.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00156
Abstract: Enzyme-catalyzed reactions sometimes display curvature in their Eyring plots in the absence of denaturation, indicative of a change in activation heat capacity. However, the effects of pH and (de)protonation on this phenomenon have remained unexplored.… read more here.
Keywords: phosphorylase geobacillus; pyrimidine nucleoside; heat; heat capacity ... See more keywords
Origin of Enzymatic Kinetic Isotope Effects in Human Purine Nucleoside Phosphorylase
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DOI: 10.1021/acscatal.7b04199
Abstract: Here we report a study of the effect of heavy isotope labeling on the reaction catalyzed by human purine nucleoside phosphorylase (hPNP) to elucidate the origin of its catalytic effect and of the enzymatic kinetic… read more here.
Keywords: purine nucleoside; human purine; isotope; enzymatic kinetic ... See more keywords
Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent
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DOI: 10.1038/s41598-021-90472-4
Abstract: E. coli purine nucleoside phosphorylase is a homohexamer, which structure, in the apo form, can be described as a trimer of dimers. Earlier studies suggested that ligand binding and kinetic properties are well described by… read more here.
Keywords: purine nucleoside; dimers forming; y160w mutant; coli purine ... See more keywords
The First Purine Nucleoside Phosphorylase Deficiency Patient Resembling IgA Deficiency and a Review of the Literature
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DOI: 10.1080/08820139.2019.1570249
Abstract: ABSTRACT Purine nucleoside phosphorylase (PNP) deficiency is a rare autosomal recessive primary immunodeficiency disorder characterized by decreased numbers of T-cells, variable B-cell abnormalities, decreased amount of serum uric acid and PNP enzyme activity. The affected… read more here.
Keywords: purine nucleoside; pnp; recurrent infections; first purine ... See more keywords
Interactions of 2,6-substituted purines with purine nucleoside phosphorylase from Helicobacter pylori in solution and in the crystal, and the effects of these compounds on cell cultures of this bacterium
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2022.2061965
Abstract: Abstract Helicobacter pylori represents a global health threat with around 50% of the world population infected. Due to the increasing number of antibiotic-resistant strains, new strategies for eradication of H. pylori are needed. In this… read more here.
Keywords: nucleoside phosphorylase; substituted purines; interactions substituted; purine nucleoside ... See more keywords
Crystallization and Preliminary X-ray Diffraction Study of Purine Nucleoside Phosphorylase from the Thermophilic Bacterium Thermus thermophilus Strain HB27
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DOI: 10.1134/s1063774518050279
Abstract: Recombinant purine nucleoside phosphorylase from the thermophilic Thermus thermophilus strain encoded by the TT_C0194 gene was purified to homogeneity. The crystallization conditions for the enzyme were found by the vapor-diffusion technique. The crystals of the… read more here.
Keywords: ray diffraction; purine nucleoside; nucleoside phosphorylase;