Structure of the Human Core Centromeric Nucleosome Complex
Sign Up to like & getrecommendations! Published in 2019 at "Current Biology"
DOI: 10.1016/j.cub.2019.06.062
Abstract: Centromeric nucleosomes are at the interface of the chromosome and the kinetochore that connects to spindle microtubules in mitosis. The core centromeric nucleosome complex (CCNC) harbors the histone H3 variant, CENP-A, and its binding proteins, CENP-C… read more here.
Keywords: cenp; nucleosome complex; centromeric nucleosome; core centromeric ... See more keywords
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-46178-y
Abstract: Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI). To address this issue, we developed graphene-oxide-coated EM… read more here.
Keywords: nucleosome complex; graphene oxide; grids enable; cryo ... See more keywords