Characterization of Impurities in Therapeutic RNAs at the Single Nucleotide Level.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04681
Abstract: The chemistry of guide RNA (gRNA) affects the performance of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing technique. However, the literature is very scarce about the study of gRNA degradation and in… read more here.
Keywords: characterization impurities; nucleotide level; chemistry; rnas single ... See more keywords
Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level
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DOI: 10.3390/v11040357
Abstract: Composed of a few hundreds of nucleotides, viroids are infectious, circular, non-protein coding RNAs able to usurp plant cellular enzymes and molecular machineries to replicate and move in their hosts. Several secondary and tertiary RNA… read more here.
Keywords: nucleotide level; viroid; rna; single nucleotide ... See more keywords
Nucleotide-level linkage of transcriptional elongation and polyadenylation
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DOI: 10.7554/elife.83153
Abstract: Alternative polyadenylation yields many mRNA isoforms whose 3’ termini occur disproportionately in clusters within 3’ UTRs. Previously, we showed that profiles of poly(A) site usage are regulated by the rate of transcriptional elongation by RNA… read more here.
Keywords: elongation; nucleotide level; transcriptional elongation; polyadenylation ... See more keywords