Quantification and Mapping of Alkylation in the Human Genome Reveal Single Nucleotide Resolution Precursors of Mutational Signatures
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DOI: 10.1021/acscentsci.2c01100
Abstract: Chemical modifications to DNA bases, including DNA adducts arising from reactions with electrophilic chemicals, are well-known to impact cell growth, miscode during replication, and influence disease etiology. However, knowledge of how genomic sequences and structures… read more here.
Keywords: damage; alkylation; single nucleotide; resolution ... See more keywords
Quantitative nucleotide resolution profiling of RNA cytidine acetylation by ac4C-seq.
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DOI: 10.1038/s41596-021-00501-9
Abstract: A prerequisite to defining the transcriptome-wide functions of RNA modifications is the ability to accurately determine their location. Here, we present N4-acetylcytidine (ac4C) sequencing (ac4C-seq), a protocol for the quantitative single-nucleotide resolution mapping of cytidine… read more here.
Keywords: ac4c seq; cytidine acetylation; nucleotide resolution;
Genome-wide analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine at single-nucleotide resolution unveils reduced occurrence of oxidative damage at G-quadruplex sites
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1022
Abstract: Abstract 8-Oxo-7,8-dihydro-2′-deoxyguanosine (OG), one of the most common oxidative DNA damages, causes genome instability and is associated with cancer, neurological diseases and aging. In addition, OG and its repair intermediates can regulate gene transcription, and… read more here.
Keywords: oxo dihydro; genome wide; single nucleotide; dihydro deoxyguanosine ... See more keywords
m1A within cytoplasmic mRNAs at single nucleotide resolution: a reconciled transcriptome-wide map.
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DOI: 10.1261/rna.067348.118
Abstract: Following synthesis, RNA can be modified with over 100 chemically distinct modifications. Recently, two studies-one by our group-developed conceptually similar approaches to globally map N1-methyladenosine (m1A) at single nucleotide resolution. Surprisingly, the studies diverged quite… read more here.
Keywords: m1a; within cytoplasmic; single nucleotide; nucleotide resolution ... See more keywords