Single‐Nucleus RNA Sequencing and Spatial Transcriptomics Reveal the Immunological Microenvironment of Cervical Squamous Cell Carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203040
Abstract: Effective treatment of advanced invasive cervical cancer remains challenging nowadays. Herein, single-nucleus RNA sequencing (snRNA-seq) and SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) technology are used to investigate the immunological microenvironment of cervical squamous cell carcinoma (CSCC),… read more here.
Keywords: immunological microenvironment; rna sequencing; microenvironment; single nucleus ... See more keywords
Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq
Sign Up to like & getrecommendations! Published in 2020 at "Nature methods"
DOI: 10.1038/s41592-020-0905-x
Abstract: Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines… read more here.
Keywords: single cell; large scale; single nucleus; cell single ... See more keywords
Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows
Sign Up to like & getrecommendations! Published in 2020 at "Genome Biology"
DOI: 10.1186/s13059-020-02048-6
Abstract: Background Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have… read more here.
Keywords: single cell; single nucleus; dissociation; nucleus rna ... See more keywords
Single-Nucleus RNA Sequencing Identifies New Classes of Proximal Tubular Epithelial Cells in Kidney Fibrosis.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Society of Nephrology : JASN"
DOI: 10.1681/asn.2020081143
Abstract: BACKGROUND Proximal tubular cells (PTCs) are the most abundant cell type in the kidney. PTCs are central to normal kidney function and to regeneration versus organ fibrosis following injury. This study used single-nucleus RNA sequencing… read more here.
Keywords: single nucleus; fibrosis; nucleus rna; kidney fibrosis ... See more keywords
Perspectives on single-nucleus RNA sequencing in different cell types and tissues
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Pathology and Translational Medicine"
DOI: 10.4132/jptm.2022.12.19
Abstract: Single-cell RNA sequencing has become a powerful and essential tool for delineating cellular diversity in normal tissues and alterations in disease states. For certain cell types and conditions, there are difficulties in isolating intact cells… read more here.
Keywords: snrna seq; rna sequencing; single nucleus; cell types ... See more keywords