Improvement of operational stability of Ogataea minuta carbonyl reductase for chiral alcohol production.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.01.016
Abstract: Directed evolution of enantio-selective carbonyl reductase from Ogataea minuta was conducted to improve the operational stability of the enzyme. A mutant library was constructed by an error-prone PCR and screened using a newly developed colorimetric… read more here.
Keywords: stability; operational stability; carbonyl reductase; ogataea minuta ... See more keywords
Expression of engineered carbonyl reductase from Ogataea minuta in Rhodococcus opacus and its application to whole-cell bioconversion in anhydrous solvents.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.07.011
Abstract: The carbonyl reductase from the methylotrophic yeast Ogataea minuta can catalyze the regio- and enantio-selective reduction of prochiral ketones to chiral alcohols, and is available for industrial manufacturing of statin drugs. We previously conducted a… read more here.
Keywords: bioconversion; organic solvents; whole cell; carbonyl reductase ... See more keywords