Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-017-1087-z
Abstract: PurposeDNA methylation is an epigenetic mechanism that plays critical roles during mammalian oocyte and preimplantation embryo development. It is achieved by adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine… read more here.
Keywords: mouse oocytes; oocytes early; superovulation; expression ... See more keywords
Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.036
Abstract: Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple… read more here.
Keywords: electrical assisted; oocytes early; microinjection; early embryos ... See more keywords
Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Human Reproduction"
DOI: 10.1093/molehr/gaab040
Abstract: Abstract Mammalian oocytes and embryos rely exclusively on maternal mRNAs to accomplish early developmental processes. Since oocytes and early embryos are transcriptionally silent after meiotic resumption, most of the synthesised maternal mRNA does not undergo… read more here.
Keywords: gene expression; oocytes early; transcription; reverse transcription ... See more keywords