Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_15
Abstract: During transcriptional elongation, RNA polymerases (RNAP) employ a stepping mechanism to translocate along the DNA template while synthesizing RNA. Optical trapping assays permit the progress of single molecules of RNA polymerase to be monitored in… read more here.
Keywords: transcriptional elongation; rna polymerase; elongation; optical trapping ... See more keywords
Optical trapping of particles by radiation forces of doughnut laser beams in the Rayleigh regime
Sign Up to like & getrecommendations! Published in 2021 at "Optical and Quantum Electronics"
DOI: 10.1007/s11082-021-02752-y
Abstract: In the present paper, we will investigate the radiation forces produced by Generalized Humbert-Gaussian beams (GHGBs), after propagating through a thin lens optical system, exerted on a dielectric sphere in the Rayleigh scattering approximation. The… read more here.
Keywords: radiation forces; radiation; particles radiation; trapping particles ... See more keywords
Optical trapping and manipulation of single particles in air: Principles, technical details, and applications
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Quantitative Spectroscopy and Radiative Transfer"
DOI: 10.1016/j.jqsrt.2018.04.027
Abstract: Abstract Trapping a single aerosol particle allows detailed investigation of its fundamental properties over extended time periods without external interferences. Optical trapping has developed into a powerful tool to perform such single-particle studies. However, trapping… read more here.
Keywords: single particles; particles air; particle; spectroscopy ... See more keywords
Optical trapping forces on Rayleigh particles by a focused Bessel-Gaussian correlated Schell-model beam
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Quantitative Spectroscopy and Radiative Transfer"
DOI: 10.1016/j.jqsrt.2019.07.015
Abstract: Abstract Optical trapping forces exerted by a focused Bessel-Gaussian correlated Schell-model (BGCSM) beam on Rayleigh dielectric spheres with different refractive indices are analyzed. The dependence of radiation forces on the transverse coherence width δ0, the… read more here.
Keywords: gaussian correlated; focused bessel; beam; optical trapping ... See more keywords
Luminescence Dynamics of Silica-Encapsulated Quantum Dots During Optical Trapping
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Physical Chemistry C"
DOI: 10.1021/acs.jpcc.6b11867
Abstract: The trade-off between photobrightening and photobleaching controls the emission stability of colloidal quantum dots. This balance is critical in optical trapping configurations, where irradiances that confine and simultaneously excite the nanocrystals in the focal region… read more here.
Keywords: dynamics silica; luminescence dynamics; optical trapping; silica encapsulated ... See more keywords
In Situ Single-Cell Surgery and Intracellular Organelle Manipulation Via Thermoplasmonics Combined Optical Trapping.
Sign Up to like & getrecommendations! Published in 2021 at "Nano letters"
DOI: 10.1021/acs.nanolett.1c04075
Abstract: Microsurgery and biopsies on individual cells in a cellular microenvironment are of great importance to better understand the fundamental cellular processes at subcellular and even single-molecular levels. However, it is still a big challenge for… read more here.
Keywords: optical trapping; single cell; intracellular organelle; cell ... See more keywords
Fringe Dielectrophoresis Nanoaperture Optical Trapping with Order of Magnitude Speed-Up for Unmodified Proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Nano letters"
DOI: 10.1021/acs.nanolett.3c00208
Abstract: Single molecule analysis of proteins in an aqueous environment without modification (e.g., labels or tethers) elucidates their biophysics and interactions relevant to drug discovery. By combining fringe-field dielectrophoresis with nanoaperture optical tweezers we demonstrate an… read more here.
Keywords: dielectrophoresis nanoaperture; nanoaperture optical; order magnitude; optical trapping ... See more keywords
Unravelling 3D Dynamics and Hydrodynamics during Incorporation of Dielectric Particles to an Optical Trapping Site.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.2c11753
Abstract: Mapping of the spatial and temporal motion of particles inside an optical field is critical for understanding and further improvement of the 3D spatio-temporal control over their optical trapping dynamics. However, it is not trivial… read more here.
Keywords: hydrodynamics; particles optical; optical trapping; trapping site ... See more keywords
Detection of Glutamate Encapsulated in Liposomes by Optical Trapping Raman Spectroscopy
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c07206
Abstract: The transmission of neuronal information is propagated through synapses by neurotransmitters released from presynapses to postsynapses. Neurotransmitters released from the presynaptic vesicles activate receptors on the postsynaptic membrane. Glutamate acts as a major excitatory neurotransmitter… read more here.
Keywords: optical trapping; synaptic vesicles; spectroscopy; glutamate ... See more keywords
Optical Trapping of Nanoparticles Using All-Silicon Nanoantennas
Sign Up to like & getrecommendations! Published in 2018 at "ACS Photonics"
DOI: 10.1021/acsphotonics.8b01250
Abstract: The ability to optically trap nanoscale particles in a reliable and noninvasive manner is emerging as an important capability for nanoscience. Different techniques have been introduced, including plasmonic nanostructures. Nano-optical tweezers based on plasmonics face… read more here.
Keywords: using silicon; microscopy; trapping nanoparticles; silicon nanoantennas ... See more keywords
Temperature Measurement in Plasmonic Nanoapertures Used for Optical Trapping
Sign Up to like & getrecommendations! Published in 2019 at "ACS Photonics"
DOI: 10.1021/acsphotonics.9b00519
Abstract: Plasmonic nanoapertures generate strong field gradients enabling efficient optical trapping of nano-objects. However, because the infrared laser used for trapping is also partly absorbed into the metal leading to Joule heating, plasmonic nano-optical tweezers face… read more here.
Keywords: trapping temperature; nano optical; temperature; plasmonic nanoapertures ... See more keywords