Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3116
Abstract: Soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors (SNAREs) are universal molecular engines that drive membrane fusion. Particularly, synaptic SNAREs mediate fast calcium‐triggered fusion of neurotransmitter‐containing vesicles with plasma membranes for synaptic transmission, the basis of all… read more here.
Keywords: optical tweezers; kinetics pathway; snare; energetics kinetics ... See more keywords
Automated Optical Tweezers Manipulation to Transfer Mitochondria from Fetal to Adult MSCs to Improve Antiaging Gene Expressions.
Sign Up to like & getrecommendations! Published in 2021 at "Small"
DOI: 10.1002/smll.202103086
Abstract: Mitochondrial dysfunction is considered to be an important factor that leads to aging and premature aging diseases. Transferring mitochondria to cells is an emerging and promising technique for the therapy of mitochondrial deoxyribonucleic acid (mtDNA)-related… read more here.
Keywords: optical tweezers; automated optical; mitochondria; transfer ... See more keywords
Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_17
Abstract: Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall… read more here.
Keywords: optical tweezers; investigate structure; dna; protein dna ... See more keywords
Introduction to Optical Tweezers.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_1
Abstract: Thirty years after their invention by Arthur Ashkin and colleagues at Bell Labs in 1986 [1], optical tweezers (or traps) have become a versatile tool to address numerous biological problems. Put simply, an optical trap… read more here.
Keywords: introduction optical; optical tweezers; biology;
Optical Tweezers-Based Measurements of Forces and Dynamics at Microtubule Ends.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_16
Abstract: Microtubules are dynamic cytoskeletal polymers that polymerize and depolymerize while interacting with different proteins and structures within the cell. The highly regulated dynamic properties as well as the pushing and pulling forces generated by dynamic… read more here.
Keywords: tweezers based; microtubule ends; optical tweezers; forces dynamics ... See more keywords
High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_8
Abstract: Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex… read more here.
Keywords: optical tweezers; high resolution; single molecule; molecule fluorescence ... See more keywords
Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_19
Abstract: The unwinding of double-stranded DNA is a frequently occurring event during the cellular processes of DNA replication, repair, and transcription. To help further investigate properties of this fundamental process as well as to study proteins… read more here.
Keywords: optical tweezers; dna; rezipping single; long dna ... See more keywords
Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, Lambda, and T4 with Optical Tweezers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_20
Abstract: Viral DNA packaging is a required step in the assembly of many dsDNA viruses. A molecular motor fueled by ATP hydrolysis packages the viral genome to near crystalline density inside a preformed prohead shell in… read more here.
Keywords: optical tweezers; dna; motor; dna packaging ... See more keywords
High-Speed Optical Tweezers for the Study of Single Molecular Motors.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_9
Abstract: Mechanical transitions in molecular motors often occur on a submillisecond time scale and rapidly follow binding of the motor with its cytoskeletal filament. Interactions of nonprocessive molecular motors with their filament can be brief and… read more here.
Keywords: optical tweezers; speed optical; tweezers study; spectroscopy ... See more keywords
How should the optical tweezers experiment be used to characterize the red blood cell membrane mechanics?
Sign Up to like & getrecommendations! Published in 2017 at "Biomechanics and Modeling in Mechanobiology"
DOI: 10.1007/s10237-017-0910-x
Abstract: Stretching red blood cells using optical tweezers is a way to characterize the mechanical properties of their membrane by measuring the size of the cell in the direction of the stretching (axial diameter) and perpendicularly… read more here.
Keywords: red blood; optical tweezers; blood; blood cell ... See more keywords
Quantifying Anticancer Drug Doxorubicin Binding to DNA using Optical Tweezers
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2018.11.2729
Abstract: | 7 Abstract As a chemotherapeutic agent heavily used since 1969, Doxorubicin’s (Doxo) mission is well known and carried out, in a variety of cancers. Doxo halts cancerous cell replication and causes cancerous cells to… read more here.
Keywords: dna; doxo; molecule; binding dna ... See more keywords