Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.10.007
Abstract: Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space… read more here.
Keywords: embryos; japanese whiting; protocols microinjection; microinjection ... See more keywords
Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Brain"
DOI: 10.1186/s13041-021-00799-2
Abstract: Immunogold labeling allows localization of proteins at the electron microscopy (EM) level of resolution, and quantification of signals. The present paper summarizes methodological issues and experiences gained from studies on the distribution of synaptic and… read more here.
Keywords: microscopy; protocols pre; pre embedding; electron microscopy ... See more keywords