Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs
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DOI: 10.1186/s13059-021-02263-9
Abstract: Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool… read more here.
Keywords: functional circrnas; optimized rna; rna targeting; crispr cas13d ... See more keywords