Optogenetic switch for controlling the central metabolic flux of Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2019.06.002
Abstract: Dynamically controlling cellular metabolism can improve a cell's yield and productivity towards a target compound. However, the application of this strategy is currently limited by the availability of reversible metabolic switches. Unlike chemical inducers, light… read more here.
Keywords: escherichia coli; flux; expression; optogenetic switch ... See more keywords
Expanding the molecular versatility of an optogenetic switch in yeast
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1029217
Abstract: In the budding yeast Saccharomyces cerevisiae, the FUN-LOV (FUNgal Light Oxygen and Voltage) optogenetic switch enables high levels of light-activated gene expression in a reversible and tunable fashion. The FUN-LOV components, under identical promoter and… read more here.
Keywords: fun lovsp; fun lov; yeast; fun ... See more keywords
Modular and Molecular Optimization of a LOV (Light–Oxygen–Voltage)-Based Optogenetic Switch in Yeast
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22168538
Abstract: Optogenetic switches allow light-controlled gene expression with reversible and spatiotemporal resolution. In Saccharomyces cerevisiae, optogenetic tools hold great potential for a variety of metabolic engineering and biotechnology applications. In this work, we report on the… read more here.
Keywords: lov; oxygen voltage; switch; fun lov ... See more keywords