Fluid shear stress applied by orbital shaking induces MG-63 osteosarcoma cells to activate ERK in two phases through distinct signaling pathways
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2017.01.004
Abstract: Fluid shear stress (FSS) induces a series of biochemical responses in osteoblasts, and this “mechanoresponse” regulates their survival, proliferation and differentiation. However, the events in cells immediately after FSS application are unclear, and how biochemical… read more here.
Keywords: orbital shaking; shear stress; method; fluid shear ... See more keywords
`Stirred not shaken!' Comparing agitation methods for permeability studies using a novel type of 96-well sandwich-plates.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2021.06.006
Abstract: In order to achieve a high sample throughput, permeation experiments are often carried out using 96-well sandwich plates. Even though agitation is regarded as important, permeation studies in 96-well format are often carried out without… read more here.
Keywords: orbital shaking; sandwich plates; agitation; using novel ... See more keywords
Miniaturized Forced Degradation of Therapeutic Proteins and ADCs by Agitation-Induced Aggregation using Orbital Shaking of Microplates.
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DOI: 10.1016/j.xphs.2021.09.027
Abstract: Microplate-based formulation screening is a powerful approach to identify stabilizing excipients for therapeutic proteins while reducing material requirements. However, this approach is sometimes not representative of studies conducted in relevant container closures. The present study… read more here.
Keywords: orbital shaking; induced aggregation; aggregation; therapeutic proteins ... See more keywords
Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis
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DOI: 10.3390/ijms22020955
Abstract: Bioreactors are increasingly implemented for large scale cultures of various mammalian cells, which requires optimization of culture conditions. Such upscaling is also required to produce red blood cells (RBC) for transfusion and therapy purposes. However,… read more here.
Keywords: orbital shaking; mechanical stress; stress induces; stress ... See more keywords