OpenSpec Enables Detecting Unexpected Modifications from Proteomics Data Generated by Orbitrap Astral Mass Spectrometer.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.5c03055
Abstract: The Orbitrap Astral mass spectrometer features outstanding speed, resolution, and sensitivity, making data-independent acquisition (DIA) the preferred method for deep profiling in shotgun proteomics. However, as for data generated by an Orbitrap Astral mass spectrometer,… read more here.
Keywords: chemistry; orbitrap astral; unexpected modifications; astral mass ... See more keywords
Isobaric Tagging and Data Independent Acquisition as Complementary Strategies for Proteome Profiling on an Orbitrap Astral Mass Spectrometer.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.4c01107
Abstract: Comprehensive global proteome profiling that is amenable to high throughput processing will broaden our understanding of complex biological systems. Here, we evaluate two leading mass spectrometry techniques, Data Independent Acquisition (DIA) and Tandem Mass Tagging… read more here.
Keywords: orbitrap astral; data independent; independent acquisition; mass ... See more keywords
Evaluation of a Prototype Orbitrap Astral Zoom Mass Spectrometer for Quantitative Proteomicsî—¸Beyond Identification Lists
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.5c00593
Abstract: Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbitrap Astral Zoom mass spectrometer (MS) prototype and compare its performance… read more here.
Keywords: mass; prototype; orbitrap astral; astral zoom ... See more keywords
Expanding the landscape of aging via orbitrap astral mass spectrometry and tandem mass tag integration
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DOI: 10.1038/s41467-025-60022-x
Abstract: Aging results in a progressive decline in physiological function due to the deterioration of essential biological processes. While proteomics offers insights into aging mechanisms, prior studies are limited in proteome coverage and lifespan range. To… read more here.
Keywords: age; tandem mass; orbitrap astral; mass tag ... See more keywords
Breaking barriers in crosslinking mass spectrometry with enhanced throughput and sensitivity using Orbitrap Astral
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DOI: 10.1038/s41467-025-64844-7
Abstract: Crosslinking mass spectrometry is an essential tool for probing protein-protein interactions and structural organization. We here compare Orbitrap Astral and Orbitrap Eclipse instruments using Cas9 crosslinked with PhoX and DSSO under standardized chromatographic and acquisition… read more here.
Keywords: orbitrap astral; spectrometry; mass spectrometry; crosslinking mass ... See more keywords