Standardization of organoid culture in cancer research.
Sign Up to like & getrecommendations! Published in 2023 at "Cancer medicine"
DOI: 10.1002/cam4.5943
Abstract: Establishing a valid in vitro model to represent tumor heterogeneity and biology is critical but challenging. Tumor organoids are self-assembled three-dimensional cell clusters which are of great significance for recapitulating the histopathological, genetic, and phenotypic… read more here.
Keywords: research; biology; organoid culture; medicine ... See more keywords
Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-66229-w
Abstract: Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D organoid cells. It costs a lot of money and consumes more time and effort… read more here.
Keywords: organoid culture; culture; bladder cancer; cancer organoid ... See more keywords
Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-83590-6
Abstract: Adenomatous polyposis coli (APC) is a tumor-suppressing protein whose inactivation triggers the formation of colorectal polyps. Numerous studies using cell lines or genetically engineered mice have revealed its role in suppressing Wnt/β-catenin signaling pathway and… read more here.
Keywords: polyposis coli; colon; organoid culture; differentiation ... See more keywords
Establishment of a bat lung organoid culture model for studying bat-derived infectious diseases
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-88621-0
Abstract: Bat is considered a natural reservoir of various important pathogens, including severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2, Ebola virus, and Nipah virus. To study these viruses’ pathogenicity and proliferation efficacy and viral tolerance mechanisms… read more here.
Keywords: organoid culture; establishment bat; biology; culture ... See more keywords
Abstract 35: Hydrogel-based microwell chip for improved organoid culture and on-chip imaging
Sign Up to like & getrecommendations! Published in 2025 at "Cancer Research"
DOI: 10.1158/1538-7445.am2025-35
Abstract: Organoids have become essential tools for modeling complex biological systems, driving significant advancements in drug screening, cancer research, and regenerative medicine. However, traditional dome-shaped organoid culture methods come with notable limitations. These include variability in… read more here.
Keywords: hydrogel based; organoid culture; microwell chip; culture ... See more keywords
Effects of hydrogel stiffness and viscoelasticity on organoid culture: a comprehensive review
Sign Up to like & getrecommendations! Published in 2025 at "Molecular Medicine"
DOI: 10.1186/s10020-025-01131-7
Abstract: As an emerging technology, organoids are promising new tools for basic and translational research in disease. Currently, the culture of organoids relies mainly on a type of unknown composition scaffold, namely Matrigel, which may pose… read more here.
Keywords: mechanical properties; organoid culture; review; stiffness viscoelasticity ... See more keywords
Enhancing organoid culture: harnessing the potential of decellularized extracellular matrix hydrogels for mimicking microenvironments
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Biomedical Science"
DOI: 10.1186/s12929-024-01086-7
Abstract: Over the past decade, organoids have emerged as a prevalent and promising research tool, mirroring the physiological architecture of the human body. However, as the field advances, the traditional use of animal or tumor-derived extracellular… read more here.
Keywords: organoid culture; extracellular matrix; enhancing organoid; culture harnessing ... See more keywords
3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2021.698292
Abstract: Lumen formation of salivary glands has been investigated using in vivo or ex vivo rudiment culture models. In this study, we used a three-dimensional (3D) salivary gland organoid culture system and demonstrated that lumen formation… read more here.
Keywords: lumen formation; gland morphogenesis; salivary gland; organoid culture ... See more keywords
Cancer organoid co-culture model system: Novel approach to guide precision medicine
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1061388
Abstract: Three-dimensional cancer organoids derived from self-organizing cancer stems are ex vivo miniatures of tumors that faithfully recapitulate their structure, distinctive cancer features, and genetic signatures. As novel tools, current cancer organoids have been well established… read more here.
Keywords: cancer organoids; organoid culture; medicine; cancer ... See more keywords
Organoid culture of bladder cancer cells
Sign Up to like & getrecommendations! Published in 2018 at "Investigative and Clinical Urology"
DOI: 10.4111/icu.2018.59.3.149
Abstract: An organoid is a miniature three-dimensional (3D), multicellular, “stem-cell derived genetically-encoded self-assembly programmed structure” which exhibits characteristics, e.g., cell-cell interactions, tissue polarity, hypoxia, drug penetration, and nutrition gradients, that recapitulate the in vivo state of… read more here.
Keywords: medicine; bladder cancer; culture bladder; cancer ... See more keywords