Organotypic culture assays for murine and human primary and metastatic-site tumors
Sign Up to like & getrecommendations! Published in 2020 at "Nature Protocols"
DOI: 10.1038/s41596-020-0335-3
Abstract: Cancer invasion and metastasis are challenging to study in vivo since they occur deep inside the body over extended time periods. Organotypic 3D culture of fresh tumor tissue enables convenient real-time imaging, genetic and microenvironmental… read more here.
Keywords: organotypic culture; primary metastatic; human primary; metastatic site ... See more keywords
HPV16-Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-33865-2
Abstract: A major risk factor for cervical cancer is persistent infection with high-risk human papillomaviruses (HPV) which can cause cervical intraepithelial neoplasia. Greater than 90% of cervical cancers develop in the transformation zone (TZ), a small… read more here.
Keywords: endocervix; transformation zone; organotypic culture; immortal cells ... See more keywords
Preserved seminiferous tubule integrity with spermatogonial survival and induction of Sertoli and Leydig cell maturation after long-term organotypic culture of prepubertal human testicular tissue
Sign Up to like & getrecommendations! Published in 2017 at "Human Reproduction"
DOI: 10.1093/humrep/dew300
Abstract: STUDY QUESTION Is an organotypic culture system able to provide the appropriate testicular microenvironment for in-vitro maturation of human immature testicular tissue (ITT)? SUMMARY ANSWER Our organotypic culture system provided a microenvironment capable of preserving… read more here.
Keywords: functionality; organotypic culture; tissue; maturation ... See more keywords
Tubular organotypic culture model of human kidney
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0206447
Abstract: Cell-culture methods that simplify the inherent complexities of the kidney have not sufficiently reproduced its true characteristics. Although reports indicate that organoid methodology surpasses traditional cell culture in terms of reproducing the nature of organs,… read more here.
Keywords: organotypic culture; human kidney; model; culture ... See more keywords
Short-Term Hypothermic Holding of Mouse Immature Testicular Tissue Does Not Alter the Expression of DNA Methyltransferases and Global DNA Methylation Level, Post-Organotypic Culture
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Endocrinology"
DOI: 10.3389/fendo.2022.854297
Abstract: Introduction Cryopreservation of immature-testicular-tissue (ITT) prior to gonadotoxic treatment, while experimental, is the only recommended option for fertility preservation in prepubertal boys. The handling and manipulation of ITT prior to banking could influence the functionality,… read more here.
Keywords: dna; organotypic culture; dna methylation; expression ... See more keywords
Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23147975
Abstract: Organotypic culture of human fetal testis has achieved fertilization-competent spermatids followed by blastocysts development. This study focuses on whether the organotypic culture of testicular tissue from infant boys with cryptorchidism could support the development of… read more here.
Keywords: culture; organotypic culture; tissue infant; testicular tissue ... See more keywords