A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-28695-w
Abstract: The coordinated action of multiple replicative helicase loading factors is needed for the licensing of replication origins prior to DNA replication. Binding of the Origin Recognition Complex (ORC) to DNA initiates the ATP-dependent recruitment of… read more here.
Keywords: dna; origin licensing; cerevisiae orc; cdc6 ... See more keywords
MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing
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DOI: 10.1038/s41467-024-51538-9
Abstract: Origin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase. However, how ORC and MCM2-7 facilitate genome-wide DNA licensing is not… read more here.
Keywords: genome wide; orc release; origin licensing; orc ... See more keywords
Temporal control of human DNA replication licensing by CDK4/6-RB signalling and chemical genetics
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DOI: 10.1038/s41467-025-63669-8
Abstract: Cyclin-dependent kinases (CDKs) coordinate DNA replication and cell division, and play key roles in tissue homeostasis, genome stability and cancer development. The first step in replication is origin licensing, when minichromosome maintenance (MCM) helicases are… read more here.
Keywords: replication; genetics; dna replication; cdk4 ... See more keywords
Efficiency and equity in origin licensing to ensure complete DNA replication.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Society transactions"
DOI: 10.1042/bst20210161
Abstract: The cell division cycle must be strictly regulated during both development and adult maintenance, and efficient and well-controlled DNA replication is a key event in the cell cycle. DNA replication origins are prepared in G1… read more here.
Keywords: replication; dna replication; origin licensing; efficiency equity ... See more keywords
Human CST suppresses origin licensing and promotes AND-1/Ctf4 chromatin association
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DOI: 10.26508/lsa.201800270
Abstract: This study suggests a novel role of the telomere-associated human CST complex in suppressing origin licensing, through interactions with MCM, while promoting the recruitment of AND-1 and DNA polymerase alpha for replisome formation. Human CTC1-STN1-TEN1… read more here.
Keywords: cst; human cst; origin licensing; replication ... See more keywords
Nucleosomes influence multiple steps during replication initiation
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DOI: 10.7554/elife.22512
Abstract: Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation. To generate distinct… read more here.
Keywords: replication initiation; activation; steps replication; origin licensing ... See more keywords
Cdc6 is sequentially regulated by PP2A-Cdc55, Cdc14, and Sic1 for origin licensing in S. cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.74437
Abstract: Cdc6, a subunit of the pre-replicative complex (pre-RC), contains multiple regulatory cyclin-dependent kinase (Cdk1) consensus sites, SP or TP motifs. In Saccharomyces cerevisiae, Cdk1 phosphorylates Cdc6-T7 to recruit Cks1, the Cdk1 phospho-adaptor in S phase,… read more here.
Keywords: cdc14; origin licensing; cdk1; cdc6 ... See more keywords